W1 - Olympus TIRF

Suitable for membrane trafficking studies.

Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] MPI-CBG LMF (MPI-CBG, 340)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope	, Inverted stand, manual IX71, manual XY stage
objectives	Olympus PlanApo 100x 1.45 TIRFM
illumination	Transmitted Light (Halogen) Fluorescence (HBO) Laser DPSS 491 nm Laser DPSS 561 nm
detection	Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um image pixel size: with 100x objective: 0.16um; 100x obj. and 2x optovar: 0.08um;
reflectors	Cairn optosplit II image splitter: two cubes: 520/15Ex DC560LP 617/73Em; 525/30 DC565LP 628/40Em Sutter filter wheel: position 0: BL HC 520/15, 1: BL HC 628/40, 2: BL HC 525/30, 3: BL HC 607/36, 4: dual emitter: Bl HC 512/23+630/91, 5: open, 6: dual emitter: Z491/561, 7: blocked, 8: BL HC LP488, 9: BL HC 617/73 microscope filter turret: position 1: for TIRF 488Ex/DC500/LP505Em, 2: for TIRF Z488Ex/DCZDC488/BP 520/40, 3: empty, 4: RFP cube, 5: dual Ex and DC for 491 and 561 laser excitation, no emitter!, 6: only DC and EM LP 575
features	custom-built TIRF condenser, manual TIRF angle adjustment
software
incubation	Stage incubator (T+CO2) + objective heater
links	[ manual] laser power values [- beam path] [ advanced system check]
inv.nr.	404504

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