W1 - Olympus TIRF

Suitable for membrane trafficking studies.

Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] MPI-CBG LMF (MPI-CBG, 340)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope	, Inverted stand, manual IX71, manual XY stage
objectives	Olympus PlanApo 100x 1.45 TIRFM Olympus UAPON 150x 1.45 TIRFM
illumination	Transmitted Light (Halogen) Fluorescence (HBO) Laser DPSS 491 nm Laser DPSS 561 nm Laser DPSS 640 nm
detection	Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um image pixel size: with 100x objective: 0.16um; 100x obj. and 2x optovar: 0.08um;
reflectors	Hamamatsu W-View image splitter: two matching dichromatic mirrors: 590LP + 590SP; four emitters: 520/15, 525/30, 617/73, 628/40 Sutter filter wheel: position 0: BL HC 520/15, 1: BL HC 628/40, 2: BL HC 525/30, 3: BL HC 607/36, 4: dual emitter: Bl HC 512/23+630/91, 5: open, 6: dual emitter: Z491/561, 7: EdgeBasic 635 LP, 8: BL HC LP488, 9: BL HC 617/73 microscope filter turret: position 1: for TIRF 488Ex/DC500/LP505Em, 2: for TIRF Z488Ex/DCZDC488/BP 520/40, 3: empty, 4: tripple DC for 491, 561 and 640 laser excitation, no emitter!, 5: dual Ex and DC for 491 and 561 laser excitation, no emitter!, 6: only DC and EM LP 575
features	custom-built TIRF condenser, manual TIRF angle adjustment
software	iQ 1.9.1
incubation	Stage incubator (T+CO2) + objective heater
links	[ manual] laser power values [ beam path] [ advanced system check]
inv.nr.	404504

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