W3 - Zeiss CCD Upright

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(following information added: objective info, camera info, darkfield condenser, software)
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|type=Microscope
 
|type=Microscope
 
|category=Wide-Field
 
|category=Wide-Field
|facility=MPI-CBG EM
+
|facility=MPI-CBG LMF
 
|building=MPI-CBG
 
|building=MPI-CBG
|room=U48
+
|room=U36
|Facility-logo=EM Facility Logo 200.jpg
+
|Facility-logo=LMF Facility Logo 200.jpg
 
|Institute-logo=Logo MPI-CBG.jpg
 
|Institute-logo=Logo MPI-CBG.jpg
 
|description=It is an upright, motorized microscope with medium sensitivity CCD camera, fitted with uniblitz shutters.
 
|description=It is an upright, motorized microscope with medium sensitivity CCD camera, fitted with uniblitz shutters.
|applications=correlative light electron microscopy/tomography (CLEM) with multi-channel imaging of fixed sample, sample screening, including CFP/Yfp FRET, image acquisition for deconvolution and DIC imaging
+
|applications=correlative light electron microscopy/tomography (CLEM) with multi-channel imaging of fixed sample, sample screening, including CFP/Yfp FRET, image acquisition for deconvolution and DIC imaging, dark field microscopy
 
|image=MPI07.jpg
 
|image=MPI07.jpg
 
|stand-name=Zeiss - Axioplan 2
 
|stand-name=Zeiss - Axioplan 2
 
|stand=Upright
 
|stand=Upright
|microscope=manual XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC
+
|microscope=manual XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC; special dark field condenser
|obj1=Zeiss Plan-Neofluar 100x 1.3 Oil
+
|obj1=Zeiss Fluar 5x 0.25
 +
|obj2=Zeiss Plan-Neofluar 10x 0.3 Ph1
 +
|obj3=Zeiss Plan-Neofluar 40x 0.75
 +
|obj4=Zeiss Achroplan 63x 0.95 W
 +
|obj5=Zeiss Plan-Apochromat 100x 1.4 Oil
 +
|obj6=Zeiss EC Plan-Neofluar 100x 1.3 Oil
 
|ill1=Transmitted Light (Halogen)
 
|ill1=Transmitted Light (Halogen)
 
|ill2=Fluorescence (HBO)
 
|ill2=Fluorescence (HBO)
|detection=Diagnostic Instruments RT SPOT monochrome camera with 1600x1200 pixels (6.7 um^2)
+
|detection=Diagnostic Instruments RT SPOT monochrome camera with 1600x1200 pixels (6.7 um^2); imaging source fire wire monochrome camera DMK 31AF03 FC 8bit with 1024 x 768 pixels (4.65um^2)
 
|reflectors=DAPI (X BP 333-383, D LP 408, M BP 432-484)<br>FITC (X BP 446-494, DLP 513, M BP 516-567)<br>TRITC ( X BP 543-554, D LP 578, M BP 579-637<br>YFP 491-509, D LP 517, M BP 519-546)<br>CFP X BP 426-446, D LP 453,M BP 465-499)<br>Brightfield, Darkfield, Phase Contrast, and DIC<br>FITC/TRITC (X DBP 474-490 & 542-566, D DBP 502-533 & 577-677< M DBP 503-533 & 581-621)
 
|reflectors=DAPI (X BP 333-383, D LP 408, M BP 432-484)<br>FITC (X BP 446-494, DLP 513, M BP 516-567)<br>TRITC ( X BP 543-554, D LP 578, M BP 579-637<br>YFP 491-509, D LP 517, M BP 519-546)<br>CFP X BP 426-446, D LP 453,M BP 465-499)<br>Brightfield, Darkfield, Phase Contrast, and DIC<br>FITC/TRITC (X DBP 474-490 & 542-566, D DBP 502-533 & 577-677< M DBP 503-533 & 581-621)
 
|features=-
 
|features=-
|software=-
+
|software=MetaVue, version 7.1.3
 
|incubation=Not available
 
|incubation=Not available
 
|link1=-
 
|link1=-

Revision as of 18:17, 22 September 2014

Suitable for correlative light electron microscopy/tomography (CLEM) with multi-channel imaging of fixed sample, sample screening, including CFP/Yfp FRET, image acquisition for deconvolution and DIC imaging, dark field microscopy.
MPI07.jpg


Directions

Logo MPI-CBG.jpg
100pxMPI-CBG LMF (MPI-CBG, U36)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope Zeiss - Axioplan 2, Upright stand, manual XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC; special dark field condenser
objectives
Zeiss Fluar 5x 0.25
Zeiss Plan-Neofluar 10x 0.3 Ph1
Zeiss Plan-Neofluar 40x 0.75
Zeiss Achroplan 63x 0.95 W
Zeiss Plan-Apochromat 100x 1.4 Oil
Zeiss EC Plan-Neofluar 100x 1.3 Oil
illumination
Transmitted Light (Halogen)
Fluorescence (HBO)
detection Diagnostic Instruments RT SPOT monochrome camera with 1600x1200 pixels (6.7 um^2); imaging source fire wire monochrome camera DMK 31AF03 FC 8bit with 1024 x 768 pixels (4.65um^2)
reflectors DAPI (X BP 333-383, D LP 408, M BP 432-484)
FITC (X BP 446-494, DLP 513, M BP 516-567)
TRITC ( X BP 543-554, D LP 578, M BP 579-637
YFP 491-509, D LP 517, M BP 519-546)
CFP X BP 426-446, D LP 453,M BP 465-499)
Brightfield, Darkfield, Phase Contrast, and DIC
FITC/TRITC (X DBP 474-490 & 542-566, D DBP 502-533 & 577-677< M DBP 503-533 & 581-621)
features -
software MetaVue, version 7.1.3
incubation Not available
links
  • [ manual]
  • [- laser power values]
  • [- beam path]
  • [ advanced system check]
inv.nr. 401536



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