WF Screening System, BIOTEC
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{{Equipment | {{Equipment | ||
|system-number=BIO23 | |system-number=BIO23 | ||
| − | |system-name=WF | + | |system-name=WF Screening System, BIOTEC |
|type=Microscope | |type=Microscope | ||
|category=Wide-Field | |category=Wide-Field | ||
|facility=Imaging@BIOTEC/CRTD | |facility=Imaging@BIOTEC/CRTD | ||
|building=BIOTEC | |building=BIOTEC | ||
| − | |room= | + | |room=242 |
|Facility-logo=Imaging@Biotec.jpg | |Facility-logo=Imaging@Biotec.jpg | ||
|Institute-logo=Biotec logo.png | |Institute-logo=Biotec logo.png | ||
| − | |description= | + | |description=It is a high-content fast widefield screening microscope capable of automated imaging of fluorescence, transmitted light and phase contrast with a large field of view. It supplies a laser-based autofocus and provides an environmental control (T, CO2, humidity) unit. Thus it is suitable for fixed as well as for live cell imaging. The stage can be moved with 100 nm precision in x, y, and z direction. An universal plate holder provides flexibility from slides up to 1536 well plates. |
| − | |applications= | + | |applications=high content screening of fluorescently labeled or unlabeled cells, either alive or fixed. |
| − | |stand-name=MD ImageXpress Micro | + | |image=WF Screening System, BIOTEC.jpg |
| + | |stand-name=MD ImageXpress Micro XLS System | ||
|stand=Inverted | |stand=Inverted | ||
|microscope=- | |microscope=- | ||
| Line 21: | Line 22: | ||
|obj6=Nikon PlanFluor 10x 0.3 WD 16 mm | |obj6=Nikon PlanFluor 10x 0.3 WD 16 mm | ||
|obj7=Nikon PlanFluor ELWD 20x 0.45 Ph1 | |obj7=Nikon PlanFluor ELWD 20x 0.45 Ph1 | ||
| − | |ill1 | + | |ill1=Transmitted Light (Nikon 100w Pillar Diascopic Illuminator) |
| − | + | |ill2=Fluorescence (Lumencor Spectra X light engine) | |
| − | | | + | |
|detection=4.66 Megapixel scientific CMOS | |detection=4.66 Megapixel scientific CMOS | ||
|reflectors=DAPI: EX 377/50 Dichroic 344-404 (R), 415-570 (T) EM 447/60<br> | |reflectors=DAPI: EX 377/50 Dichroic 344-404 (R), 415-570 (T) EM 447/60<br> | ||
| − | |||
CFP: EX 438/24 Dichroic 426-450 (R), 467-600 (T) EM 483/32<br> | CFP: EX 438/24 Dichroic 426-450 (R), 467-600 (T) EM 483/32<br> | ||
Texas Red: EX 562/40 Dichroic 530-585 (R), 601-800 (T) EM 624/40<br> | Texas Red: EX 562/40 Dichroic 530-585 (R), 601-800 (T) EM 624/40<br> | ||
| − | GFP: EX 472/30 Dichroic 442-488 (R), 502-730 (T) EM 520/35 | + | GFP: EX 472/30 Dichroic 442-488 (R), 502-730 (T) EM 520/35<br> |
| − | |features= | + | Cy5: EX 628/40 Dichroic 594-651 (R), 669-726 (T) EM 692/40<br> |
| − | |software= | + | Customized Cy5 will follow |
| + | |features=hardware autofocus | ||
| + | |software=MetaXpress Imaging and Analysis software<br> | ||
| + | AcuityXpress Analysis and Visualization software<br> | ||
| + | MDCStore database and Microsoft SQL Express | ||
|incubation=Cage incubator (T+CO2) | |incubation=Cage incubator (T+CO2) | ||
| − | |inv= | + | |inv=25007452 |
}} | }} | ||
Latest revision as of 16:13, 15 February 2017
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[edit] Directions
[edit] Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c
[edit] Details
| microscope | MD ImageXpress Micro XLS System, Inverted stand, - | ||
| objectives | |||
| illumination | |||
| detection | 4.66 Megapixel scientific CMOS | ||
| reflectors | DAPI: EX 377/50 Dichroic 344-404 (R), 415-570 (T) EM 447/60 CFP: EX 438/24 Dichroic 426-450 (R), 467-600 (T) EM 483/32 | ||
| features | hardware autofocus | ||
| software | MetaXpress Imaging and Analysis software AcuityXpress Analysis and Visualization software | ||
| incubation | Cage incubator (T+CO2) | ||
| links |
| ||
| inv.nr. | 25007452 |
