WF Screening System, BIOTEC

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|Facility-logo=Imaging@Biotec.jpg
 
|Facility-logo=Imaging@Biotec.jpg
 
|Institute-logo=Biotec logo.png
 
|Institute-logo=Biotec logo.png
|description=High content widefield screening microscope capable of automated imaging of fluorescence, transmitted light and phase contrast with a large field of view. It comes with an environmental control to adjust temperature, CO2 and humidity and is therfore suitable for both fixed and live cell imaging. The stage can be moves with 100 nm precision in x, y, and z direction. An universal plate holder provides flexibility from slides up to 1536 well plates.
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|description=High-content, very fast widefield screening microscope capable of automated imaging of fluorescence, transmitted light and phase contrast with a large field of view. It has a laser-based autofocus and is capable of on-the-fly deconvolution. It comes with an environmental control to adjust temperature, CO2 and humidity and is therfore suitable for both fixed and live cell imaging. The stage can be moved with 100 nm precision in x, y, and z direction. An universal plate holder provides flexibility from slides up to 1536 well plates.  
 
|applications=high content screening of fluorescently labeled or unlabeled cells, either alive or fixed.
 
|applications=high content screening of fluorescently labeled or unlabeled cells, either alive or fixed.
 
|stand-name=MD ImageXpress Micro XL System
 
|stand-name=MD ImageXpress Micro XL System

Revision as of 11:10, 2 December 2013

Suitable for high content screening of fluorescently labeled or unlabeled cells, either alive or fixed..
[[image:|250px]]


Directions

Biotec logo.png
Imaging@Biotec.jpgImaging@BIOTEC/CRTD (BIOTEC, 242)

Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

Details

microscope MD ImageXpress Micro XL System, Inverted stand, -
objectives
Nikon PlanApo Lambda 2x 0.1 WD 8.5 mm
Nikon PlanApo Lambda 4x 0.2 WD 20 mm
Nikon PlanApo Lambda 10x 0.45 WD 4 mm
Nikon PlanApo Lambda 20x 0.75 WD 1 mm
Nikon PlanApo Lambda 40x 0.95
Nikon PlanFluor 10x 0.3 WD 16 mm
Nikon PlanFluor ELWD 20x 0.45 Ph1
illumination
Transmitted Light (Nikon 100w Pillar Diascopic Illuminator)
Fluorescence (Lumencor Spectra X light engine)
detection 4.66 Megapixel scientific CMOS
reflectors DAPI: EX 377/50 Dichroic 344-404 (R), 415-570 (T) EM 447/60

CFP: EX 438/24 Dichroic 426-450 (R), 467-600 (T) EM 483/32
Texas Red: EX 562/40 Dichroic 530-585 (R), 601-800 (T) EM 624/40
GFP: EX 472/30 Dichroic 442-488 (R), 502-730 (T) EM 520/35
Cy5: EX 628/40 Dichroic 594-651 (R), 669-726 (T) EM 692/40
Customized Cy5 will follow

features -
software MetaXpress Imaging and Analysis software

MDCStore database and Microsoft SQL Express"MetaXpress Imaging and Analysis software<br /> MDCStore database and Microsoft SQL Express" cannot be used as a page name in this wiki.

incubation Cage incubator (T+CO2)
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. 0



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