Warner Instruments TB-3 CCD Thermal Insert

Revision as of 16:20, 28 August 2008

Overview

Warner Instruments TB-3 CCD with custom-made rack and 96 well plate adapter, plus the OKO-LAB metal dish

The TB-3 is available in two versions:

• CCD - made for 35 mm dishes and Warner RC-40 chambers
• CS - made for chamber slides up to 1 x 3'

One main advantage of the Warner heating and cooling incubation system is the cooling of small volumes of medium directly on the microscope. Due to the design (water-cooled Peltier element) it is quite light-weight and works on fragile Z-stages as well. The official working range is 5 - 50 ° celsius. However, real 5 °C could never be reached on our side. In best case we got it down to 6,5 °C.

This manual gives some hints about its usage.

Requirements

• Warner Instruments TB-3 CCD Thermal Insert
• Prior NZ200CE NanoScan Z-stage - or different/custom-made insert for other stages
• OKO-LAB glass-bottom metal dish, model RA-35-18-2000-06 (recommended for best performance)

Manual

1. Initial steps

1. Setup before imaging

1. Handling while imaging