Zeiss LSM 510 Tile Scan
From BioDIP
(Difference between revisions)
| Line 5: | Line 5: | ||
how to to do a proper tile scan using multi-time module | how to to do a proper tile scan using multi-time module | ||
| + | *set up imaging conditions | ||
| + | *save scan configuration | ||
| + | *generate database file for the tile scan | ||
| + | *open multi-time > multiple locations | ||
| + | *define configuration > load configuration | ||
| + | *a) edit locations > tile mark corners > overlap 20% > create tile locations | ||
| + | *b) edit locations > tile > define tile numbers > overlap 20% > create tile locations | ||
| + | *start time | ||
| + | *wait for acquisition and saving | ||
| + | *select resulting window All_(filename)_Sum | ||
| + | *tile utilities > correlate > correlation range 3 um | ||
| + | *wait for correlation | ||
| + | *save the result | ||
[[Image:Zeiss_lsm510_filemenu.jpg|thumb]] | [[Image:Zeiss_lsm510_filemenu.jpg|thumb]] | ||
Revision as of 16:15, 20 June 2008
manual
Zeiss LSM 510
how to to do a proper tile scan using multi-time module
- set up imaging conditions
- save scan configuration
- generate database file for the tile scan
- open multi-time > multiple locations
- define configuration > load configuration
- a) edit locations > tile mark corners > overlap 20% > create tile locations
- b) edit locations > tile > define tile numbers > overlap 20% > create tile locations
- start time
- wait for acquisition and saving
- select resulting window All_(filename)_Sum
- tile utilities > correlate > correlation range 3 um
- wait for correlation
- save the result
