Zeiss LSM 780 NLO - DZNE M1
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{{Equipment | {{Equipment | ||
− | |system-number=DZNE- | + | |system-number=DZNE-M1 |
|system-name=Zeiss LSM 780 NLO | |system-name=Zeiss LSM 780 NLO | ||
|type=Microscope | |type=Microscope | ||
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|room=208 | |room=208 | ||
|Facility-logo=DZNE-Imaging-logo2-v6.png | |Facility-logo=DZNE-Imaging-logo2-v6.png | ||
− | |Institute-logo=Logo-DZNE. | + | |Institute-logo=Logo-DZNE.jpg |
|description=Zeiss LSM 780 NLO | |description=Zeiss LSM 780 NLO | ||
*Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation. | *Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation. | ||
*Single photon point scanning confocal system with two conventional PMTs and one spectral GaAsP detector and transmitted light detector. Motorized stage for multi-point acquisition | *Single photon point scanning confocal system with two conventional PMTs and one spectral GaAsP detector and transmitted light detector. Motorized stage for multi-point acquisition | ||
− | |applications=imaging of thick specimens and fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan | + | |applications=imaging of thick specimens and fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan |
|image=02 LSM780NLO DZNE.jpg | |image=02 LSM780NLO DZNE.jpg | ||
|stand-name=Zeiss - Axio Examiner.Z1 | |stand-name=Zeiss - Axio Examiner.Z1 | ||
|stand=Upright | |stand=Upright | ||
|microscope=motorized XY stage, motorized Z-drive, | |microscope=motorized XY stage, motorized Z-drive, | ||
− | |obj1=Zeiss | + | |obj1=Zeiss Plan-Apochromat 10x 0.45 |
− | |obj2=Zeiss Plan-Apochromat | + | |obj2=Zeiss Plan-Apochromat 20x 0.8 |
− | |obj3=Zeiss Plan-Apochromat | + | |obj3=Zeiss Plan-Apochromat 40x 0,95 korr. |
− | |obj4=Zeiss Plan-Apochromat | + | |obj4=Zeiss LD LCI Plan-Apochromat 25x/0.8 Imm Korr DIC |
+ | |obj5=Zeiss LD W Plan-Apochromat 20x 1.0 Korr. VIS-IR | ||
|obj6=Zeiss W Plan-Apochromat 20x/1.0 | |obj6=Zeiss W Plan-Apochromat 20x/1.0 | ||
|ill1=Fluorescence (Metal Halide, HXP, 120W) | |ill1=Fluorescence (Metal Halide, HXP, 120W) | ||
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|ill8=Laser Multiphoton Chameleon VisionII 690-1064nm | |ill8=Laser Multiphoton Chameleon VisionII 690-1064nm | ||
|detection='''Single photon detection:''' 2 confocal PMTs with enhanced detection in the red range, GaAsP spectral detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting <br> | |detection='''Single photon detection:''' 2 confocal PMTs with enhanced detection in the red range, GaAsP spectral detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting <br> | ||
− | '''2 photon detection:''' Two-channel non-descanned GaAsP detector (BiG) set up for emission spectra of EGFP (500-550nm) and RFP (575-610nm) | + | '''2 photon detection:''' Two-channel non-descanned GaAsP detector (BiG) set up for emission spectra of EGFP (500-550nm) and RFP (575-610nm)<br> |
+ | Two-channel non-descanned detector (NDD) set up for emission spectra of DAPI (447/60 nm) and Cy5 (700/75 nm) | ||
|reflectors=Zeiss set 49: DAPI<br> | |reflectors=Zeiss set 49: DAPI<br> | ||
Zeiss set 38: GFP<br> | Zeiss set 38: GFP<br> |
Latest revision as of 16:46, 28 January 2016
[edit] Directions
[edit] Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc14ffd3aa3e44c3
[edit] Details
microscope | Zeiss - Axio Examiner.Z1, Upright stand, motorized XY stage, motorized Z-drive, | ||
objectives | |||
illumination | |||
detection | Single photon detection: 2 confocal PMTs with enhanced detection in the red range, GaAsP spectral detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting 2 photon detection: Two-channel non-descanned GaAsP detector (BiG) set up for emission spectra of EGFP (500-550nm) and RFP (575-610nm) | ||
reflectors | Zeiss set 49: DAPI Zeiss set 38: GFP | ||
features | simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan | ||
software | Zen "black" 2011 | ||
incubation | Not available | ||
links |
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inv.nr. | AIM System 2504000174 |