Zeiss LSM 780 NLO - DZNE M1

From BioDIP
(Difference between revisions)
Jump to: navigation, search
m (Whites moved page Zeiss LSM 780 NLO - DZNE 02 to Zeiss LSM 780 NLO - DZNE M1: name change)
 
(3 intermediate revisions by 2 users not shown)
Line 1: Line 1:
 
{{Equipment
 
{{Equipment
|system-number=DZNE-02
+
|system-number=DZNE-M1
 
|system-name=Zeiss LSM 780 NLO
 
|system-name=Zeiss LSM 780 NLO
 
|type=Microscope
 
|type=Microscope
Line 21: Line 21:
 
|obj3=Zeiss Plan-Apochromat 40x 0,95 korr.
 
|obj3=Zeiss Plan-Apochromat 40x 0,95 korr.
 
|obj4=Zeiss LD LCI Plan-Apochromat 25x/0.8 Imm Korr DIC
 
|obj4=Zeiss LD LCI Plan-Apochromat 25x/0.8 Imm Korr DIC
 +
|obj5=Zeiss LD W Plan-Apochromat 20x 1.0 Korr. VIS-IR
 
|obj6=Zeiss W Plan-Apochromat 20x/1.0
 
|obj6=Zeiss W Plan-Apochromat 20x/1.0
 
|ill1=Fluorescence (Metal Halide, HXP, 120W)
 
|ill1=Fluorescence (Metal Halide, HXP, 120W)
Line 30: Line 31:
 
|ill8=Laser Multiphoton Chameleon VisionII 690-1064nm
 
|ill8=Laser Multiphoton Chameleon VisionII 690-1064nm
 
|detection='''Single photon detection:''' 2 confocal PMTs with enhanced detection in the red range, GaAsP spectral detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting <br>
 
|detection='''Single photon detection:''' 2 confocal PMTs with enhanced detection in the red range, GaAsP spectral detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting <br>
'''2 photon detection:''' Two-channel non-descanned GaAsP detector (BiG) set up for emission spectra of EGFP (500-550nm) and RFP (575-610nm)
+
'''2 photon detection:''' Two-channel non-descanned GaAsP detector (BiG) set up for emission spectra of EGFP (500-550nm) and RFP (575-610nm)<br>
 
Two-channel non-descanned detector (NDD) set up for emission spectra of DAPI (447/60 nm) and Cy5 (700/75 nm)
 
Two-channel non-descanned detector (NDD) set up for emission spectra of DAPI (447/60 nm) and Cy5 (700/75 nm)
 
|reflectors=Zeiss set 49: DAPI<br>
 
|reflectors=Zeiss set 49: DAPI<br>

Latest revision as of 16:46, 28 January 2016

  • Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation.
  • Single photon point scanning confocal system with two conventional PMTs and one spectral GaAsP detector and transmitted light detector. Motorized stage for multi-point acquisition"Zeiss LSM 780 NLO
  • Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation.
  • Single photon point scanning confocal system with two conventional PMTs and one spectral GaAsP detector and transmitted light detector. Motorized stage for multi-point acquisition" cannot be used as a page name in this wiki.

Suitable for imaging of thick specimens and fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan.
02 LSM780NLO DZNE.jpg


[edit] Directions

Logo-DZNE.jpg
DZNE-Imaging-logo2-v6.pngDZNE - Imaging Platform (DZNE, 208)

[edit] Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc14ffd3aa3e44c3

[edit] Details

microscope Zeiss - Axio Examiner.Z1, Upright stand, motorized XY stage, motorized Z-drive,
objectives
Zeiss Plan-Apochromat 10x 0.45
Zeiss Plan-Apochromat 20x 0.8
Zeiss Plan-Apochromat 40x 0,95 korr.
Zeiss LD LCI Plan-Apochromat 25x/0.8 Imm Korr DIC
Zeiss LD W Plan-Apochromat 20x 1.0 Korr. VIS-IR
Zeiss W Plan-Apochromat 20x/1.0
illumination
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (Halogen)
Laser Diode 405 nm
Laser Argon Multiline 458, 488, 514 nm
Laser DPSS 561 nm
Laser HeNe 633 nm
Laser Multiphoton Chameleon VisionII 690-1064nm
detection Single photon detection: 2 confocal PMTs with enhanced detection in the red range, GaAsP spectral detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting

2 photon detection: Two-channel non-descanned GaAsP detector (BiG) set up for emission spectra of EGFP (500-550nm) and RFP (575-610nm)
Two-channel non-descanned detector (NDD) set up for emission spectra of DAPI (447/60 nm) and Cy5 (700/75 nm)

reflectors Zeiss set 49: DAPI

Zeiss set 38: GFP
Zeiss set 43: Cy3

features simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan
software Zen "black" 2011
incubation Not available
links
  • manual
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. AIM System 2504000174



Personal tools