Biopolis Dresden Imaging Platform

ApoTome1 3rd floor upright CRTD (Zeiss)

It is designed for routine work on fixed samples. Optical sectioning can be obtained on fixed samples by the use of the Apotome.

Category

Widefield, ApoTome

Location

CRTD, 3rd floor, LMF CMCB, room 3.325

Suitable for Specimen
Fixed samples
Tissue sections
Suitable for Technique
Large area tile/mosaic scans
Microscope Stand

Zeiss Axio Imager.Z1, upright stand
manual objective revolver, motorized z-focus

Objectives

2.5x/0.12 Fluar, Air, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 8.7 mm
Thread: M27
Tube Length: infinity
Objective ID: CMCB 176 2.5x/0.12 Zeiss
Order Number: 420120-9901-000

10x/0.45 Plan-Apochromat, Air, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 2 mm
Thread: M27
Tube Length: infinity
Objective ID: CMCB 83 10x/0.45 Zeiss
Order Number: 420640-9800-000

20x/0.8 Plan-Apochromat, Air, DIC, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 0.55 mm
Nomarski Prism: EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940)
Thread: M27
Tube Length: infinity
Objective ID: CMCB 84 20x/0.8 Zeiss
Order Number: 420650-9901-000

40x/0.95 Plan-Apochromat, Air, DIC, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 0.25 mm
Nomarski Prism: EC PN and Fluar 40/1,3 III; PA 40/0,95 III (426944)
Thread: M27
Tube Length: infinity
Objective ID: CMCB 123 40x/0.95 Zeiss
Order Number: 420660-9970-000

40x/0.75 Achroplan, Water, DIC, Zeiss

Immersion: Water
Working Distance: 2.1 mm
Nomarski Prism: Achroplan 40/0,75 W III (444451)
Thread: M27
Tube Length: infinity
Objective ID: CMCB 104 40x/0.75 Water dipping Zeiss
Order Number: 440090

63x/1.2 C-Apochromat, Water, DIC, Zeiss

Immersion: Water
Collar: coverslip correction
Coverslip Thickness: 0.17
Working Distance: 0.28 mm at cover glass 0.17 mm
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: CMCB 93 63x/1.2 Water Zeiss
Order Number: 440668
Condenser

BF, DF, DIC II, DIC III, Ph1, Ph2, Ph3
NA 0.9, working distance 1.2 mm, motorized

Fluorescence Filter Cubes

motorized revolver

polarizer (set Analysator)

polarizer cube for DIC/Polarization

Zeiss, set Analysator

DAPI (set F46-000)

single cube for DAPI, Alexa 350

  • BP 387/11 (BP 381-392), excitation filter, bandpass, AHF
  • LP 400, dichromatic mirror, longpass, AHF
  • BP 460/50 (BP 435-485), emission filter, bandpass, AHF

Zeiss, set F46-000

GFP (set F46-000)

single cube for Alexa488/GFP, FITC

  • BP 470/40 (BP 450-490), excitation filter, bandpass, AHF
  • LP 495, dichromatic mirror, longpass, AHF
  • BP 525/36 (BP 507-543), emission filter, bandpass, AHF

Zeiss

Cy3 (set F46-004)

single cube for Alexa555/Cy3/RFP, TRITC

  • BP 590/33 (BP 573-606), emission filter, bandpass, AHF
  • LP 565, dichromatic mirror, longpass, AHF
  • BP 545/25 (BP 533-558), excitation filter, bandpass, AHF

Zeiss, set F46-004

Cy5 (set F46-006)

single cube for Alexa633/Cy5

  • BP 640/30 (BP 625-655), excitation filter, bandpass, Zeiss
  • LP 660, dichromatic mirror, longpass, Zeiss
  • BP 700/75 (BP 663-738), emission filter, bandpass, Zeiss

Zeiss, set F46-006

YFP (set F46-003)

single cube for YFP

  • BP 500/20 (BP 490-510), excitation filter, bandpass, AHF
  • LP 515, dichromatic mirror, longpass, Leica
  • BP 535/30 (BP 520-550), emission filter, bandpass, AHF

Zeiss, set F46-003

GFP/RFP (set 24)

double cube for GFP/RFP

  • BP 485/20; BP 578/14 (BP 475-495; BP 571-585), excitation filter, double bandpass, Zeiss
  • BP 528/65, LP 600 (BP 495-560, LP 600), double dichromatic mirror, double bandpass, Zeiss
  • BP 528/25; LP 610 (BP 515-540; LP 610), emission filter, double bandpass, Zeiss

Zeiss, set 24

Transmitted Illumination

Lamp (Halogen, 100W)

Fluorescence Illumination

HXP 120C

Zeiss, Metal halide lamp, 120W, integrated
Camera

Zeiss AxioCam MRc, Color CCD camera, dexel size 6.45 µm

port top port
magnification 1x
bit depth 12 bit
sensor size 8.9 x 6.7 mm
maximum frame size 1388 x 1040 dexel
maximum full chip frame rate 12.86 fps
Serial number 206081617

Zeiss AxioCam MRm, Monochrome CCD camera, dexel size 6.45 µm

port left side port
magnification 1x
bit depth 12 bit
sensor size 8.9 x 6.7 mm
maximum frame size 1388 x 1040 dexel
maximum full chip frame rate 12.86 fps
Serial number 112082065
XY Stage

motorized stage

Märzhäuser, Typ: EK 75x50 SZ; Serial-No: 07121212 with

  • holder for 1 slide
Structured Illumination

ApoTome1, Zeiss

structured illumination for optical sectioning

Computer

P Z820 Workstation

Windows 7 Ultimate (dual boot 32/64 bit) operating system

  • 8 x 8 GB RAM
  • Intel Xeon E5-2643 (2x) CPU
  • 2 x 24" display
  • APOT1-3FLOOR-CR
Software

Zeiss ZEN blue, V 2012

  • 1983806491
  • 3D VisArt
  • Experiment Designer
  • Extended Focus
  • Measurement
  • Multi channel acquisition
  • Tiles
  • Time Lapse
  • Z-stack

Zeiss Axiovision,

Additional Information
  • Inventory Number 163533000
  • SIP Number 3512001681
  • Contact for Service Raik Stäber (0151/42112278)
  • DRESDEN concept ID 1289