Biopolis Dresden Imaging Platform

Light Microscopy

Avoiding Pitfalls

Cromey, DW
Avoiding Twisted Pixels: Ethical Guidelines for the Appropriate Use and Manipulation of Scientific Digital Images
Sci Eng Ethics. 2010 Dec;16(4):639-67

Pearson, H
The good, the bad and the ugly
Nature. 2007 May 10;447(7141):138-40

Brown, CM
Fluorescence microscopy - avoiding the pitfalls
J Cell Sci. 2007 May 15;120(Pt 10):1703-5

Eisenstein, Michael
Something to see
Nature. 2006 Oct 26;443(7114):1017-21

Pawley, J
The 39 Steps: A Cautionary Tale about “quantatative” 3D Fluorescence Microscopy
Biotechniques. 2000 May;28(5):884-6, 888


Becker, W
Fluorescence lifetime imaging – techniques and applications
J Microsc. 2012 Aug;247(2):119-36

Weber, M and Huisken J
Light sheet microscopy for real-time developmental biology
Curr Opin Genet Dev. 2011 Oct;21(5):566-72

Truong TV, Supatto W, Koos DS, Choi JM, Fraser SE
Deep and fast live imaging with two-photon scanned light-sheet microscopy
Nat Methods. 2011 Jul 17;8(9):757-60

Min W, Freudiger CW, Lu S, Xie XS
Coherent nonlinear optical imaging: beyond fluorescence microscopy
Annu Rev Phys Chem. 2011;62:507-30

Hell, SW
Microscopy and its focal switch
Nat Methods. 2009 Jan;6(1):24-32

Evans, CL and Xie XS
Coherent Anti-Stokes Raman Scattering Microscopy: Chemical Imaging for Biology and Medicine
Annu Rev Anal Chem. 2008;1:883-909

Mazza D, Braeckmans K, Cella F, Testa I, Vercauteren D, Demeester J, De Smedt SS, Diaspro A
A New FRAP/FRAPa Method For 3-D Diffusion Measurements Based On Multi-photon Excitation Microscopy
Biophys J. 2008 Oct;95(7):3457-69

Göbel W, Kampa BM, Helmchen F.
Imaging cellular network dynamics in three dimensions using fast 3D laser scanning
Nat Methods. 2007 Jan;4(1):73-9

Botcherby EJ, Juskaitis R, Booth MJ, Wilson T
Aberration-free optical refocusing in high numerical aperture microscopy
Opt Lett. 2007 Jul 15;32(14):2007-9

Donnert G, Eggeling C, Hell SW
Major signal increase in fluorescence microscopy through dark-state relaxation
Nat Methods. 2007 Jan;4(1):81-6

Frohn JT, Knapp HF, Stemmer A
Three-dimensional resolution enhancement in fluorescence microscopy by harmonic excitation
Opt Lett. 2001 Jun 1;26(11):828-30



Fluorescent Proteins

Shcherbakova DM, Verkhusha VV
Near-infrared fluorescent proteins for multicolor in vivo imaging
Nat Methods. 2013 Aug;10(8):751-4

Shcherbakova DM, Subach OM, Verkhusha VV
Red fluorescent proteins: advanced imaging applications and future design
Angew Chem Int Ed Engl. 2012 Oct 22;51(43):10724-38

Wu B, Piatkevich KD, Lionnet T, Singer RH, Verkhusha VV
Modern fluorescent proteins and imaging technologies to study gene expression, nuclear localization, and dynamics
Curr Opin Cell Biol. 2011 Jun;23(3):310-7

Goedhart J, van Weeren L, Hink MA, Vischer NO, Jalink K, Gadella TW Jr
Bright cyan fluorescent protein variants identified by fluorescence lifetime screening
Nat Methods. 2010 Feb;7(2):137-9

Shcherbo D et al.
Far-red fluorescent tags for protein imaging in living tissues
Biochem J. 2009 Mar 15;418(3):567-74

Kredel S et al.
Optimized and Far-Red-Emitting Variants of Fluorescent Protein eqFP611
Chem Biol. 2008 Mar;15(3):224-33

Shaner NC, Patterson GH, Davidson MW
Advances in fluorescent protein technology
J Cell Sci. 2007 Dec 15;120(Pt 24):4247-60

Koushik SV, Chen H, Thaler C, Puhl HL 3rd, Vogel SS
Cerulean, Venus, and VenusY67C FRET Reference Standards
Biophys J. 2006 Dec 15;91(12):L99-L101

Eisenstein M
Helping cells to tell a colorful tale
Nat Methods. 2006 Aug; 3(8): 647 - 655

Technical Tests

Cole, RW, Brown CM, Jinadasa T
Measuring and interpreting point spread functions to determine confocal microscope resolution and ensure quality control
Nat Protoc. 2011 Nov 10;6(12):1929-41

Murray, JM, Appleton PL, Swedlow JR, Waters JC
Evaluating performance in three-dimensional fluorescence microscopy
J Micros. 2007 Dec;228(Pt 3):390-405

Zucker, RM
Quality Assessment of Confocal Microscopy Slide Based Systems: Performance
Cytometry A. 2006 Jul;69(7):659-76

Zucker, RM
Quality Assessment of Confocal Microscopy Slide-Based Systems: Instability
Cytometry A. 2006 Jul;69(7):677-90

Zucker, RM
Reliability of confocal microscopy spectral imaging systems: Use of multispectral beads
Cytometry A. 2007 Mar;71(3):174-89

Zucker, RM, Price O
Evaluation of confocal microscopy system performance
Cytometry. 2001 Aug 1;44(4):273-94