ApoTome2 inverse CRTD (Zeiss)
Our inverted ApoTome2 Microscope with hardware autofocus, colour and monochrome cameras is designed for routine work on fixed sample. Optical sectioning can be obtained on fixed samples by the use of the ApoTome2 slider.
Category
Widefield, ApoTome
Location
CRTD, basement south, LMF CMCB, room -1.124
Suitable for Specimen
Suitable for Technique
Microscope Stand
Objectives
5x/0.16 Plan-Apochromat, Air, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 12.1 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 82 5x/0.16 Zeiss |
| Order Number: | 420630-9900-000 |
10x/0.3 EC Plan-Neofluar, Air, Ph1, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 5.2 mm |
| Nomarski Prism: | EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 139 10x/0.3 Ph1 Zeiss |
| Order Number: | 420341-9911-000 |
20x/0.4 LD Plan-Neofluar, Air, Ph2, Zeiss
| Immersion: | Air |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 7.9 mm |
| Nomarski Prism: | LD PN 20/0,40 II (426954), PlasDIC for LD PN 20/0,40 Korr (444504) |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | CMCB 35 LD 20x/0.4 Ph2 Zeiss |
| Order Number: | 441341-9970-000 |
20x/0.8 Plan-Apochromat, Air, DIC, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.55 mm |
| Nomarski Prism: | EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 159 20x/0.8 Zeiss |
| Order Number: | 420650-9901-000 |
Condenser
Fluorescence Filter Cubes
motorized revolver
DAPI (set F26-510)
single cube for Alexa 350, DAPI
- BP 390/18 (BP 381-399), excitation filter, bandpass, AHF
- LP 416, dichromatic mirror, longpass, AHF
- BP 460/60 (BP 430-490), emission filter, bandpass, AHF
Zeiss, set F26-510
GFP (set 38 HE)
single cube for Alexa488/GFP, FITC
- BP 470/40 (BP 450-490), excitation filter, bandpass, Chroma
- LP 495, dichromatic mirror, longpass, Chroma
- BP 525/50 (BP 500-550), emission filter, bandpass, Chroma
Zeiss, set 38 HE
Cy3 (set 43 HE)
single cube for Alexa555/Cy3/RFP, TRITC
- BP 550/25 (BP 525-575), excitation filter, bandpass, Zeiss
- LP 570, dichromatic mirror, longpass, Zeiss
- BP 605/70 (BP 570-640), emission filter, bandpass, Zeiss
Zeiss, set 43 HE
Cy5 (set 43 HE)
single cube for Alexa633/Cy5
- BP 620/60 (BP 590-650), excitation filter, bandpass, Zeiss
- LP 660, dichromatic mirror, longpass, Zeiss
- BP 700/75 (BP 663-738), emission filter, bandpass, Zeiss
Zeiss
CFP (set 47 HE)
single cube for CFP
- BP 436/25 (BP 424-449), excitation filter, bandpass, Zeiss
- LP 455, dichromatic mirror, longpass, Zeiss
- BP 480/40 (BP 460-500), emission filter, bandpass, Zeiss
Zeiss, set 47 HE
YFP (set 47 HE)
single cube for YFP
- BP 500/20 (BP 490-510), excitation filter, bandpass, AHF
- LP 515, dichromatic mirror, longpass, Leica
- BP 535/30 (BP 520-550), emission filter, bandpass, AHF
Zeiss
Calibration (set 47 HE)
single cube for YFP
Zeiss
polarizer (set Analysator)
polarizer cube for DIC/Polarization
Zeiss, set Analysator
Transmitted Illumination
Lamp (Halogen, 100W)
Fluorescence Illumination
HXP 120C
Zeiss, Metal halide lamp, 120W, manual
470nm
LED
590nm
LED
Camera
Zeiss AxioCam HRc, Color CCD camera, dexel size 6.45 µm
| port | left side port |
| magnification | 1x |
| bit depth | 12 bit |
| sensor size | 8.9 x 6.7 mm |
| maximum frame size | 1388 x 1040 dexel |
| maximum full chip frame rate | 12 fps at full chip size |
Zeiss AxioCam MRm, Monochrome CCD camera, dexel size 6.45 µm
| port | right side port |
| magnification | 1x |
| bit depth | 12 bit |
| sensor size | 8.9 x 6.7 mm |
| maximum frame size | 1388 x 1040 dexel |
| maximum full chip frame rate | 12.86 fps |
XY Stage
Hardware Autofocus
Zeiss Definite Focus.1
Structured Illumination
ApoTome2, Zeiss
structured illumination for optical sectioning
Computer
Software
Additional Information