Live Cell AF6000 CRTD (Leica)
Our Leica AFLX6000 is designed for routine work and dedicated for LiveCell imaging in XYZ. The incubator allows control on temperature and CO2, and the motorized stage allows multiple-position imaging. The simple LASAF interface makes it straight forward to work with. A double micromanipulator allows precise manipulation of the sample.
Category
Widefield
Location
CRTD, basement south, LMF CMCB, room -1.123
Suitable for Specimen
Suitable for Technique
Microscope Stand
Objectives
40x/1.25 HC PL APO CS, Oil, DIC, Leica
| Immersion: | Oil |
| Collar: | NA correction |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.1 mm |
| Nomarski Prism: | D |
| Thread: | M25 |
| Tube Length: | infinity |
| Objective ID: | CMCB 19 40x/1.25-0.75 Oil Leica corr. |
| Order Number: | 11506251 |
Condenser
Fluorescence Filter Cubes
motorized revolver
DAPI (set A)
single cube for Alexa 350, DAPI
- BP 360/40 (BP 340-380), emission filter, bandpass, Leica
- LP 400, dichromatic mirror, longpass, Leica
- LP 425, emission filter, longpass, Leica
Leica, set A
GFP (set L5)
single cube for Alexa488/GFP, FITC
- BP 480/40 (BP 460-500), excitation filter, bandpass, Leica
- LP 505, dichromatic mirror, longpass, Leica
- BP 527/30 (BP 512-542), emission filter, bandpass, Leica
Leica, set L5
GFP (set L5)
single cube for Alexa488/GFP, FITC
- BP 470/40 (BP 450-490), excitation filter, bandpass, AHF
- LP 495, dichromatic mirror, longpass, AHF
- LP 500, emission filter, longpass, AHF
Leica
Cy3 (set N2.1)
single cube for Alexa555/Cy3/RFP, TRITC
- BP 537/45 (BP 515-560), excitation filter, bandpass, Leica
- LP 580, dichromatic mirror, longpass, Leica
- LP 590, emission filter, longpass, Leica
Leica, set N2.1
Cy5 (set F36-523)
single cube for Alexa633/Cy5
- BP 628/40 (BP 608-648), excitation filter, bandpass, Chroma
- LP 660, dichromatic mirror, longpass, AHF
- BP 692/40 (BP 672-712), emission filter, bandpass, AHF
Leica, set F36-523
YFP (set F46-003)
single cube for YFP
- BP 500/20 (BP 490-510), excitation filter, bandpass, AHF
- LP 515, dichromatic mirror, longpass, Leica
- BP 535/30 (BP 520-550), emission filter, bandpass, AHF
Leica, set F46-003
polarizer (set Analysator)
polarizer cube for DIC/Polarization
Leica, set Analysator
Transmitted Illumination
Lamp (Halogen, 100W)
Fluorescence Illumination
HXP 120C
Leica, Metal halide lamp, 120W, integrated
Camera
Cascade II 512 EMCCD, Monochrome EMCCD camera, dexel size 16 µm
| port | bottom port |
| bit depth | 16 bit |
| sensor size | 8.2 x 8.2 mm |
| maximum frame size | 512 x 512 dexel |
| maximum full chip frame rate | 38.5 fps |
Leica DFC 350 FX, Monochrome CCD camera, dexel size 6.45 µm
| port | left side port |
| bit depth | 12 bit |
| sensor size | 9 x 6.7 mm |
| maximum frame size | 1392 x 1040 dexel |
| maximum full chip frame rate | 21.07 - 10.54 fps |
XY Stage
Incubation
Computer
Software
Additional Information