Biopolis Dresden Imaging Platform

LSI Macro Confocal CRTD (Leica)

It is an upright super zoom macro confocal microscope with a tuneable spectral detector. It has an 16x optical zoom. The systems offers 4 laser lines and a spectral detector. It has a large working distance and a field of view up to 16mm.

Location

CRTD, basement south, LMF CMCB, room -1.120

Suitable for Specimen
Fixed samples
Microscope Stand

Leica DMRE, upright stand
manual objective revolver, motorized z-focus

Additional magnification: 16x optical zoom, motorized

Additional z-focus device: Leica SuperZ stage

Objectives

1x/ PLAN APOCHROMAT, Air, Leica

Immersion: Air
Tube Length: infinity
Objective ID: CMCB 81 1x Leica
Order Number: 10447176

5x/0.5 PLAN APOCHROMAT, Air, Leica

Immersion: Air
Tube Length: infinity
Objective ID: CMCB 80 5x/0.5 Leica
Order Number: 10447243
Fluorescence Filter Cubes

manual revolver

DAPI (set A)

single cube for Alexa 350, DAPI

  • BP 360/40 (BP 340-380), excitation filter, bandpass, Leica
  • LP 400, dichromatic mirror, longpass, Leica
  • LP 425, emission filter, longpass, Leica

Leica, set A

DAPI (set D)

single cube for Alexa 350, DAPI

  • BP 390/70 (BP 355-425), excitation filter, bandpass, Leica
  • LP 455, dichromatic mirror, longpass, Leica
  • LP 470, emission filter, longpass, Leica

Leica, set D

GFP (set F36-497)

single cube for Alexa488/GFP, FITC

  • BP 472/30 (BP 457-487), excitation filter, bandpass, AHF
  • LP 495, dichromatic mirror, longpass, AHF
  • LP 496, emission filter, longpass, AHF

Leica, set F36-497

Cy3 (set N2.1)

single cube for Alexa555/Cy3/RFP, TRITC

  • BP 537/45 (BP 515-560), excitation filter, bandpass, Leica
  • LP 585, dichromatic mirror, longpass, Leica
  • LP 590, emission filter, longpass, Leica

Leica, set N2.1

Transmitted Illumination

Lamp (Halogen, 100W)

Fluorescence Illumination

HXP 120C

Leica, Metal halide lamp, 120W, integrated

405nm pulsed

Laser Diode, integrated

488nm

Laser DPSS, integrated

532nm

Laser HeNe, integrated

635nm

Laser Diode, integrated
XY Stage

motorized stage

Leica LSI mot. Scanning Stage Kit m. LSTEP with

  • multiwell plate holder
  • variable holder for slides and 3.5cm dishes
Scan Head

SPE

conventional scanner

Internal detectors:

  • 1 spectral detection PMT
  • T-PMT
Computer

HP

Windows XP Professional operating system

  • 2 x 2 GB RAM
  • Intel Core i7-860 CPU
  • 2 x 19" display
  • LSI-MACRO-CRTD
Software

Leica LAS AF,

  • 1319892
  • Acquisition
  • Base
  • Live Data Mode
Additional Information
  • Inventory Number 71163/0
  • SIP Number SN: 5220000356
  • Contact for Service Hr. Schreiner (0172/9401123)
  • DRESDEN concept ID 1296