Biopolis Dresden Imaging Platform

LSM 700 inverse BIOTEC (Zeiss)

It is designed for routine work and is dedicated for high resolution imaging in XYZ. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with.

Category

Laser scanning confocal

Location

BIOTEC, 2nd floor, LMF CMCB, room 227

Suitable for Specimen
Fixed samples
Tissue sections
Suitable for Technique
Multi wellplate imaging
Large area tile/mosaic scans
Microscope Stand

Zeiss Axiovert 200M, inverted stand
motorized objective revolver, motorized z-focus

Objectives

10x/0.45 Plan-Apochromat, Air, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 2 mm
Nomarski Prism: Planapo 10/0,45 II (1045-073)
Thread: M27
Tube Length: infinity
Objective ID: CMCB 195 10x/0.45 Zeiss
Order Number: 420640-9900-000

20x/0.8 Plan-Apochromat, Air, DIC, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 0.55 mm
Nomarski Prism: EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940)
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: CMCB 103 20x/0.8 Zeiss
Order Number: 440640-9903-000

40x/1.3 Plan-Neofluar, Oil, DIC, Zeiss

Immersion: Oil
Coverslip Thickness: 0.17
Working Distance: 0.21 mm
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: CMCB 42 40x/1.3 Oil Zeiss
Order Number: 1056-602 (1083-997)

40x/1.2 C-Apochromat, Water, DIC, Zeiss

Immersion: Water
Collar: coverslip correction
Coverslip Thickness: 0.17
Working Distance: 0.28 mm at cover glass 0.17 mm
Nomarski Prism: CA 40/1,2 W; LD CA 40/1,1 W III (426948)
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: CMCB 106 40x/1.2 Water Zeiss
Order Number: 441757-9970-000

63x/1.4 Plan-Apochromat, Oil, DIC, Zeiss

Immersion: Oil
Coverslip Thickness: 0.17
Working Distance: 0.19 mm
Nomarski Prism: PA 63/1,4 HC II (426952), PA 63/1,40 III (426957)
Thread: M27
Tube Length: infinity
Objective ID: CMCB 66 63x/1.4 Oil Zeiss
Order Number: 420782-9900-000

100x/1.4 Plan-Apochromat, Oil, DIC, Zeiss

Immersion: Oil
Coverslip Thickness: 0.17
Working Distance: 0.17 mm
Nomarski Prism: PA 100/1,4 HC II (426949), PA 40x/1,30 III (426965)
Thread: M27
Tube Length: infinity
Objective ID: CMCB 68 100x/1.4 Oil Zeiss
Order Number: 420792-9900-000

5x/0 APO, Air, DIC, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Thread: M27
Tube Length: infinity
Objective ID: CMCB 107 APO Calibration Zeiss
Condenser

BF, DIC II, DIC III, Ph1, Ph2, Ph3
NA 0.55, working distance 26 mm, motorized

Fluorescence Filter Cubes

motorized revolver

DAPI ()

single cube for Alexa 350, DAPI

  • BP 387/11 (BP 381-392), excitation filter, bandpass, AHF
  • LP 400, dichromatic mirror, longpass, AHF
  • BP 460/50 (BP 435-485), emission filter, bandpass, AHF

Zeiss

GFP ()

single cube for Alexa488/GFP, FITC

  • BP 480/30 (BP 465-495), excitation filter, bandpass
  • LP 505, dichromatic mirror, longpass
  • BP 535/40 (BP 515-555), emission filter, bandpass

Zeiss

Dsred ()

single cube for DsRed, mCherry/TexasRed

  • BP 545/10 (BP 540-550), excitation filter, bandpass
  • LP 555, dichromatic mirror, longpass
  • BP 600/70 (BP 565-635), emission filter, bandpass

Zeiss

Additional Filters


  • SP 490, emission filter, shortpass, Zeiss
  • SP 555, emission filter, shortpass, Zeiss
  • SP 640, emission filter, shortpass, Zeiss
  • BP 523/65 (BP 490-555), emission filter, bandpass, Zeiss


  • LP 490, emission filter, longpass, Zeiss
  • LP 560, emission filter, longpass, Zeiss
  • BP 588/55 (BP 560-615), emission filter, bandpass, Zeiss
  • LP 640, emission filter, longpass, Zeiss
Transmitted Illumination

Lamp (Halogen, 100W)

Fluorescence Illumination

HXP 120C

Zeiss, Metal halide lamp, 120W, manual

405nm pulsed

Laser Diode, integrated

488nm

Laser DPSS, integrated

555nm

Laser DPSS, integrated

639nm

Laser DPSS, integrated
XY Stage

motorized stage

Märzhäuser Scan IM 130x100 DC, Art-No: 90-25-422-000/432028-9901, SN: 11020881

Scan Head

LSM 700

conventional scanner

Internal detectors:

  • 2 confocal PMT (filter)
  • T-PMT
Computer

FUJITSU ESPRIMO P7936

Windows 7 Ultimate (64 bit) operating system

  • 4 x 2 GB RAM
  • Intel Core 2 Duo E8400 CPU
  • ATI FirePro V5800 (1GB) grafic card
  • 30" display
  • LSM700-inv-biotec
Software

Zeiss ZEN black 2011, V 8.1.0.484, SP3

  • dongle: 1149774004
  • 3D
  • FRAP
  • Tiles
Additional Information
  • Inventory Number 301200000
  • SIP Number 2601000539
  • Contact for Service Hr. Raik Stäber (0151/42112278)
  • DRESDEN concept ID 1298