Biopolis Dresden Imaging Platform

SP5 I upright BIOTEC (Leica)

Our Leica TCS SP5 I confocal setup is designed for working on living sample and dedicated for high resolution imaging in XYZ. The upright configuration and the dipping lenses make it optimal for embryo development studies. An incubator allows long timelapse with living samples. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with.

Category

Laser scanning confocal

Location

BIOTEC, 2nd floor, LMF CMCB, room 220

Suitable for Specimen
Live samples
Fixed samples
Tissue sections
Suitable for Technique
Spectral unmixing
Microscope Stand

Leica DM 6000, upright stand
motorized objective revolver, motorized z-focus

Additional z-focus device: Leica SuperZ stage

Objectives

10x/0.4 HC PL APO CS, Air, DIC, Leica

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 2.2 mm
Nomarski Prism: A
Thread: M25
Tube Length: infinity
Objective ID: CMCB 01 10x/0.4 Leica
Order Number: 11506285

20x/0.5 HC APO L U-V-I, Water, DIC, Leica

Immersion: Water
Working Distance: 3.5 mm
Nomarski Prism: D1
Thread: M25
Tube Length: infinity
Objective ID: CMCB 03 20x/0.5 Water dipping Leica
Order Number: 11506147

20x/0.7 HC PL APO CS, Air, DIC, Leica

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 0.59 mm
Thread: M25
Tube Length: infinity
Objective ID: CMCB 98 20x/0.7 Leica
Order Number: 11506513

40x/0.75 HC PL APO, Air, DIC, Leica

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 0.28 mm
Nomarski Prism: D
Thread: M25
Tube Length: infinity
Objective ID: CMCB 04 40x/0.75 Leica
Order Number: 11506173
Inventory Number: 181299000

40x/0.8 HC APO L, Water, DIC, Leica

Immersion: Water
Working Distance: 3.3 mm
Nomarski Prism: D1
Thread: M25
Tube Length: infinity
Objective ID: CMCB 11 40x/0.8 Water dipping Leica
Order Number: 11506155

63x/0.9 HC APO L U-V-I, Water, DIC, Leica

Immersion: Water
Working Distance: 2.2 mm
Nomarski Prism: D1
Thread: M25
Tube Length: infinity
Objective ID: CMCB 05 63x/0.9 Water dipping Leica
Order Number: 11506148

63x/1.2 HC PL APO lambda blue, Water, DIC, Leica

Immersion: Water
Collar: coverslip correction
Coverslip Thickness: 0.17
Working Distance: 0.22 mm
Nomarski Prism: D
Thread: M25
Tube Length: infinity
Objective ID: CMCB 06 63x/1.2 Water Leica corr.
Order Number: 11506213

63x/1.4 HC PL APO lambda blue, Oil, DIC, Leica

Immersion: Oil
Collar: NA correction
Coverslip Thickness: 0.17
Working Distance: 0.1 mm
Nomarski Prism: E
Thread: M25
Tube Length: infinity
Objective ID: CMCB 31 63x/1.4-0.6 Oil Leica corr.
Order Number: 11506192

100x/1.47 HCX PL APO, Oil, DIC, Leica

Immersion: Oil
Collar: temperature and coverslip correction
Coverslip Thickness: 0.17
Working Distance: 0.09 mm
Thread: M25
Tube Length: infinity
Objective ID: CMCB 162 100x/1.47 Oil Leica corr.
Order Number: 11506318

100x/1.4 HC PL APO, Oil, DIC, Leica

Immersion: Oil
Coverslip Thickness: 0.17
Working Distance: 0.13 mm
Thread: M25
Tube Length: infinity
Objective ID: CMCB 169 100x/1.4 Oil Leica
Order Number: 11506372
Condenser

BF, DIC I, DIC II, DIC III
NA 0.9, working distance 1.2 mm, motorized

Fluorescence Filter Cubes

motorized revolver

DAPI (set A)

single cube for DAPI, Alexa 350

  • BP 360/40 (BP 340-380), excitation filter, bandpass, Leica
  • LP 400, dichromatic mirror, longpass, Leica
  • LP 425, emission filter, longpass, Leica

Leica, set A

GFP (set L5)

single cube for Alexa488/GFP, FITC

  • BP 480/40 (BP 460-500), excitation filter, bandpass, Leica
  • LP 505, dichromatic mirror, longpass, Leica
  • BP 527/30 (BP 512-542), emission filter, bandpass, Leica

Leica, set L5

Dsred (set F46-005)

single cube for DsRed, mCherry/TexasRed

  • LP 570, dichromatic mirror, longpass, AHF
  • BP 620/60 (BP 590-650), emission filter, bandpass, AHF
  • BP 545/30 (BP 530-560), excitation filter, bandpass, AHF

Leica, set F46-005

polarizer (set Analysator)

polarizer cube for DIC/Polarization

Leica, set Analysator

Transmitted Illumination

Lamp (Halogen, 100W)

Fluorescence Illumination

HXP 120C

Leica, Metal halide lamp, 120W, integrated

405nm pulsed

Laser Diode, integrated

458, 477, 488, 496, 514nm multiline

Laser Argon, integrated

561nm

Laser DPSS, integrated

633nm

Laser HeNe, integrated
XY Stage

motorized stage

Märzhäuser EK 14 mot. 14C DM 600, SN: 05021155

Scan Head

SP5

conventional scanner

Internal detectors:

  • T-PMT
  • 2 spectral detection HyD
  • 3 confocal PMT
Incubation

splitable cage incubator

LSI

Feature:

  • CO2

  • Heating

  • Humidity

Computer

HP Z620 Workstation

Windows 7 Professional (64 bit) operating system

  • 4 x 4 GB RAM
  • Intel Xeon E5-2650 CPU
  • NVIDIA QUADRO 600 grafic card
  • 30" display
  • SP5I-UPR-BIOTEC
Software

Leica LAS AF, V 2.7.3

  • 2500205, 1331435
  • 3D
  • Acquisition
  • Base
  • Colocalization
  • Dye Finder
  • FRAP
  • FRET
  • Live Data Mode
Additional Information
  • Inventory Number 146330000
  • SIP Number SN: 5100000340
  • Contact for Service Hr. Schreiner (0172/9401123)
  • DRESDEN concept ID 1301