Biopolis Dresden Imaging Platform

[B CUBE] Ti-E with Spinning Disk (NIKON)

Nikon Ti-E TIRF/Superresolution/SpinningDisk

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Booking System
Category

Spinning disk

Location

B CUBE, MIM Facility CMCB, room E22

Suitable for Specimen
Cleared samples
Fixed samples
Live samples
Organoids
Tissue sections
Whole organisms
Whole organs
Suitable for Technique
3D imaging
Large area tile/mosaic scans
Multi wellplate imaging
Multiple positions
Timelapse imaging
Microscope Stand

NIKON Eclipse Ti-E, inverted stand
motorized objective revolver, motorized z-focus

Additional magnification: 1x / 1.5x manual zoom lens

Additional z-focus device: Mad City Labs Nano Drive

Objectives

60x/0.95 CFI PlanApo Lambda, Air, DIC, NIKON

Immersion: Air
Collar: coverslip correction
Coverslip Thickness: 0.11-0.23
Working Distance: 0.2 – 0.11 mm
Thread: M25
Tube Length: infinity
Objective ID: B CUBE Nikon 60x/0.95 Plan Apo Lambda

60x/1.27 Plan Apo IR, Water, DIC, NIKON

Immersion: Water
Collar: coverslip correction
Coverslip Thickness: 0.15-0.19
Working Distance: 0.18 – 0.16 mm
Thread: M25
Tube Length: infinity
Objective ID: B CUBE Nikon 60x/1.27 W Plan Apo IR

100x/1.49 SR Apochromat, Oil, DIC, NIKON

Immersion: Oil
Collar: temperature and coverslip correction
Coverslip Thickness: 0.13-0.20
Working Distance: 0.12 mm
Thread: M25
Tube Length: infinity
Objective ID: B CUBE Nikon 100x/1.49 Oil SR Apo TIRF

20x/0.75 Plan Apochromat VC, Air, DIC, NIKON

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 0.1 mm
Thread: M25
Tube Length: infinity
Objective ID: B CUBE Nikon 20x/0.75 Plan Apo VC

60x/1.2 Plan Apochromat VC, Water, DIC, NIKON

Immersion: Water
Collar: coverslip correction
Coverslip Thickness: 0.15-0.18
Working Distance: 0.31 – 0.28 mm
Thread: M25
Tube Length: infinity
Objective ID: B CUBE Nikon 60xA/1.20 W Plan Apo VC

40x/0.95 CFI PlanApo Lambda, Air, DIC, NIKON

Immersion: Air
Collar: coverslip correction
Coverslip Thickness: 0.11-0.23
Working Distance: 0.25 – 0.17 mm
Thread: M25
Tube Length: infinity
Objective ID: B CUBE Nikon 40x/0.95 Plan Apo Lambda
Condenser

NA 0.72, working distance 13 mm, manual

Transmitted Illumination

Metal halide lamp (Nikon D-LH/LC Halogen Lamp)

Fluorescence Illumination

Laser 405 nm

Agilent, Laser, 20 mW, integrated

Laser 488 nm

Agilent, Laser, 80 mW, integrated

Laser 561 nm

Agilent, Laser, 80 mW, integrated

Laser 647 nm

Agilent, Laser, 125 mW, integrated
Camera

Andor iXon Ultra 888, Monochrome EMCCD camera, dexel size 13 µm

port right side port
magnification 1x
bit depth 16 bit
sensor size 13.3 x 13.3 mm
maximum frame size 1024 x 1024 dexel
maximum full chip frame rate 26 fps
XY Stage

motorized stage

Nikon TI-S-ER with

  • holder for 1 slide
  • multiwell plate holder
  • petri dish holder
  • universal disholder
Hardware Autofocus

NIKON Perfect Focus System

Scan Head

Yokogawa, CSU-X1

  • max. spinning disk rotation speed: 1500 - 5000 rpm
  • maximum scanning speed: 1000 fps
  • scan head field of view size: 10 mm x 7 mm
  • physical pinhole diameter: 50 µm
  • physical pinhole spacing: 253 µm
Incubation

Stage inset

Peltier-based sample holder for slides or dishes, Warner Instruments

Feature:

  • Heating

  • Cooling

Additional Equipment

stage heating/cooling

Additional Information