Biopolis Dresden Imaging Platform

SteMi Discovery CRTD (Zeiss)

It is an upright stereo microscope with a large-chip color camera. It's suitable for transmitted and reflected bright-field, and for fluorescence imaging.

Suitable for It's particularly suitable for imaging of fixed, stained histological samples..

Category

Stereo / zoom microscope

Location

CRTD, basement south, LMF CMCB, room -1.121

Suitable for Specimen
Fixed samples
Tissue sections
Microscope Stand

Zeiss SteReo, upright stand
manual objective revolver, manual z-focus

Additional magnification: 20x optical zoom, motorized

Objectives

0.6x/ Plan Apo S, Air, Zeiss

Immersion: Air
Working Distance: 81 mm
Tube Length: infinity
Objective ID: CMCB 78 0.6x Zeiss
Order Number: 435202

1.5x/ Plan Apo S, Air, Zeiss

Immersion: Air
Working Distance: 30 mm
Tube Length: infinity
Objective ID: CMCB 79 1.5x Zeiss
Order Number: 435226
Fluorescence Filter Cubes

manual revolver

DAPI (set 58)

single cube for Alexa 350, DAPI

  • BP 390/70 (BP 355-425), excitation filter, bandpass, Zeiss
  • LP 440, dichromatic mirror, longpass, Zeiss
  • LP 470, emission filter, longpass, Zeiss

Zeiss, set 58

GFP (set 38)

single cube for Alexa488/GFP, FITC

  • BP 470/40 (BP 450-490), excitation filter, bandpass, Chroma
  • LP 495, dichromatic mirror, longpass, Chroma
  • BP 525/50 (BP 500-550), emission filter, bandpass, Chroma

Zeiss, set 38

Cy3 (set 43)

single cube for Alexa555/Cy3/RFP, TRITC

  • BP 545/25 (BP 533-558), excitation filter, bandpass, Zeiss
  • LP 570, dichromatic mirror, longpass, Zeiss
  • BP 605/70 (BP 570-640), emission filter, bandpass, Zeiss

Zeiss, set 43

GFP/RFP (set F56-019V20)

double cube for GFP/RFP

  • BP 480/20; BP 567/25 (BP 470-490; BP 555-575), excitation filter, bandpass, AHF
  • BP 522/52, 637/83 (BP 496-548, 595-678), double dichromatic mirror, double bandpass, AHF
  • BP 523/45; BP 618/56 (BP 500-545; BP 590-646), emission filter, bandpass, AHF

Zeiss, set F56-019V20

Transmitted Illumination

Lamp (KL 2500 LCD-24V, 2500W)

Fluorescence Illumination

HXP 120C

Zeiss, Metal halide lamp, 120W, manual
Camera

Spot insight color IN1420, Color CCD camera, dexel size 7.4 µm

port top port
bit depth 14 bit
sensor size 15.2 x 15.2 mm
maximum frame size 2048 x 2048 dexel
maximum full chip frame rate 5 fps
XY Stage

Zeiss Transillumination base 300

Computer

DELL Precision Workstation 390

Windows 7 Professional (32 bit) operating system

  • 2 x 2 GB RAM
  • Intel Core 2 Duo E6600 CPU
  • 19" display
  • LMF-3
Software

Diagnoscics Instruments SPOT Basic Software, V 5.1, 5678

  • test1234567890
Additional Equipment

Ringlight

Additional Information
  • Inventory Number 162036000
  • DRESDEN concept ID 1304