Biopolis Dresden Imaging Platform

[B CUBE] Ti-E with TIRF/FRAP (NIKON)

Nikon Ti-E TIRF/FRAP

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Booking System
Category

Widefield, TIRF

Location

B CUBE, MIM Facility CMCB, room E22

Suitable for Specimen
Cleared samples
Fixed samples
Live samples
Molecules at the surface of a cover slip
protein droplets
Tissue sections
Suitable for Technique
Large area tile/mosaic scans
Multi wellplate imaging
Multiple positions
Photo manipulation (PA/FRAP/FLIP/FLAP/...)
Timelapse imaging
Microscope Stand

NIKON Eclipse Ti-E, inverted stand
motorized objective revolver, motorized z-focus

Additional magnification: 1x / 1.5x manual zoom lens

Objectives

10x/0.3 Plan Fluor, Air, DIC, NIKON

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 16 mm
Thread: M25
Tube Length: infinity
Objective ID: B CUBE Nikon 10x/0.3 Plan Fluor

40x/0.95 CFI PlanApo Lambda, Air, DIC, NIKON

Immersion: Air
Collar: coverslip correction
Coverslip Thickness: 0.11-0.23
Working Distance: 0.25 – 0.17 mm
Thread: M25
Tube Length: infinity
Objective ID: B CUBE Nikon 40x/0.95 Plan Apo Lambda

60x/1.27 Plan Apo IR, Water, DIC, NIKON

Immersion: Water
Collar: coverslip correction
Coverslip Thickness: 0.15-0.19
Working Distance: 0.18 – 0.16 mm
Thread: M25
Tube Length: infinity
Objective ID: B CUBE Nikon 60x/1.27 W Plan Apo IR

100x/1.49 SR HP Apo, Oil, DIC, NIKON

Immersion: Oil
Collar: temperature and coverslip correction
Coverslip Thickness: 0.13-0.20
Working Distance: 0.12 mm
Thread: M25
Tube Length: infinity
Objective ID: B CUBE Nikon 100x/1.49 Oil SR HP Apo TIRF

60x/1.49 Apochromat, Oil, DIC, NIKON

Immersion: Oil
Collar: temperature and coverslip correction
Coverslip Thickness: 0.13-0.21
Working Distance: 0.12 mm
Thread: M25
Tube Length: infinity
Objective ID: B CUBE Nikon 60x/1.49 Oil Apo TIRF
Condenser

NA 0.52, working distance 30 mm, manual

Transmitted Illumination

Metal halide lamp (Nikon D-LH/LC Halogen Lamp)

Fluorescence Illumination

Laser 405 nm

Agilent, Laser, 20 mW, integrated

Laser 488 nm

Agilent, Laser, 80 mW, integrated

Laser 561 nm

Agilent, Laser, 80 mW, integrated

Laser 647 nm

Agilent, Laser, 125 mW, integrated

SpectraX 395/25 nm LED

Lumencor, LED, 295 mW, integrated

SpectraX 440/20 nm LED

Lumencor, LED, 256 mW, integrated

SpectraX 470/24 nm LED

Lumencor, LED, 196 mW, integrated

SpectraX 510/25 nm LED

Lumencor, LED, 62 mW, integrated

SpectraX 550/15 nm LED

Lumencor, LED, 260 mW, integrated

SpectraX 575/25 nm LED

Lumencor, LED, 310 mW, integrated

SpectraX 640/30 nm LED

Lumencor, LED, 231 mW, integrated
Camera

Andor iXon Ultra 897, Monochrome EMCCD camera, dexel size 16 µm

port left side port
magnification 1x
bit depth 16 bit
sensor size 8.2 x 8.2 mm
maximum frame size 512 x 512 dexel
maximum full chip frame rate 56 fps
XY Stage

motorized stage

Nikon TI-S-ER with

  • holder for 1 slide
  • multiwell plate holder
  • petri dish holder
  • universal disholder
Hardware Autofocus

NIKON Perfect Focus System

Incubation

Objective heater

Objective Heater, Okolab

Feature:

  • Heating

Additional Equipment

Objective Heater

Additional Information