WF Laser Microdissection CRTD (Zeiss)
It is a laser microdissection system consisting of an inverted fluorescent microscope with a motorized stage and two cameras (b/w and color)
Suitable for the manipulation of biological samples with a UV laser and the collection of cut material. It can be used for tissue sections, live cell selection and manipulations of whole organisms. Moreover it is a fully working imaging system.
Category
Widefield, Microdissection
Location
CRTD, basement south, LMF CMCB, room -1.121
Suitable for Specimen
Suitable for Technique
Microscope Stand
Objectives
5x/0.25 Fluar, Air, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 12.5 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 164 5x/0.25 Zeiss |
| Order Number: | 420130-9900-000 |
10x/0.3 EC Plan-Neofluar, Air, DIC, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 5.2 mm |
| Nomarski Prism: | EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 61 10x/0.3 Zeiss |
| Order Number: | 420340-9901-000 |
20x/0.4 LD Plan-Neofluar, Air, Ph2, Zeiss
| Immersion: | Air |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 7.9 mm at cover glass 0.75 mm |
| Nomarski Prism: | LD PN 20/0,40 II (426954), LD PN 40/0,6 (444505) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 165 LD 20x/0.4 Ph2 Zeiss |
| Order Number: | 421351-9970-000 |
40x/1.3 EC Plan-Neofluar, Oil, DIC, Zeiss
| Immersion: | Oil |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.21 mm |
| Nomarski Prism: | EC PN and Fluar 40/1,3 III; PA 40/0,95 III (426944) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 87 40x/1.3 Oil Zeiss |
| Order Number: | 440654-9902-000 |
40x/0.6 LD Plan-Neofluar, Air, DIC, Zeiss
| Immersion: | Air |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 2.9 mm at cover glass 0.75 mm |
| Nomarski Prism: | LD PN 40/0,60 II (426955), LD PN 40/0,6 (444505) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 166 LD 40x/0.6 Zeiss |
| Order Number: | 421360-9970-000 |
150x/1.35 Plan-Apochromat, Glycerine, DIC, Zeiss
| Immersion: | Glycerine |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.17 mm at cover glass 0.16 mm |
| Nomarski Prism: | PA 150x/1,35 III (426968-9000) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 167 150x/1.35 Glycerine Zeiss |
| Order Number: | 420792-9970-000 |
Condenser
Fluorescence Filter Cubes
motorized revolver
DAPI ()
single cube for Alexa 350, DAPI
- BP 367/11 (BP 362-373), excitation filter, bandpass, AHF
- LP 405, dichromatic mirror, longpass, AHF
- BP 447/60 (BP 417-477), emission filter, bandpass, AHF
Zeiss
GFP ()
single cube for Alexa488/GFP, FITC
- BP 470/40 (BP 450-490), excitation filter, bandpass, AHF
- LP 488, dichromatic mirror, longpass, AHF
- BP 525/50 (BP 500-550), emission filter, bandpass, AHF
Zeiss
GFP (set 09)
single cube for Alexa488/GFP, FITC
- BP 470/40 (BP 450-490), excitation filter, bandpass, Zeiss
- LP 510, dichromatic mirror, longpass, Zeiss
- LP 515, emission filter, longpass, Zeiss
Zeiss, set 09
Cy3 (set 09)
single cube for Alexa555/Cy3/RFP, TRITC
- BP 524/25 (BP 512-537), excitation filter, bandpass, AHF
- LP 561, dichromatic mirror, longpass, AHF
- BP 605/70 (BP 570-640), emission filter, bandpass, AHF
Zeiss
Cy5 (set 09)
single cube for Alexa633/Cy5
- BP 640/30 (BP 625-655), excitation filter, bandpass, Zeiss
- LP 635, dichromatic mirror, longpass, Zeiss
- BP 690/50 (BP 665-715), emission filter, bandpass, Zeiss
Zeiss
Calibration (set 09)
single cube for Alexa633/Cy5
Zeiss
Transmitted Illumination
Lamp (Halogen, 100W)
Fluorescence Illumination
HXP 120C
Zeiss, Metal halide lamp, 120W, integrated
355nm
Laser Diode
Camera
AxioCam Icc 1, Color CCD camera, dexel size 4.65 µm
| port | left side port |
| bit depth | 8 bit |
| sensor size | 6.3 x 4.8 mm |
| maximum frame size | 1388 x 1040 dexel |
| maximum full chip frame rate | 15.38 fps |
XY Stage
Extra Photo Manipulation
Computer
Software
Additional Information