Biopolis Dresden Imaging Platform

WF Leica inverse CRTD (Leica)

The inverted Leica microscope is designed for routine work on fixed fluorescent sample.

The instrument is especially suitable for multicolor fluorescent samples due to high sensitivity b/w camera.

Category

Widefield

Location

BIOTEC, 2nd floor, LMF CMCB, room 225

Suitable for Specimen
Fixed samples
Tissue sections
Suitable for Technique
Multi wellplate imaging
Microscope Stand

Leica DMI 4000, inverted stand
manual objective revolver, motorized z-focus

Additional z-focus device: Leica SuperZ stage

Objectives

5x/0.15 HCX PL FLUOTAR, Air, Leica

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 13.7 mm
Thread: M25
Tube Length: infinity
Objective ID: CMCB 173 5x/0.15 Leica
Order Number: 11506224

10x/0.3 HC PL S-Apo, Air, DIC, Leica

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 10 mm
Thread: M25
Tube Length: infinity
Objective ID: CMCB 75 10x/0.3 Leica
Order Number: 11506289

20x/1.4 HC PL FLUOTAR L, Air, DIC, Leica

Immersion: Air
Collar: coverslip correction
Coverslip Thickness: 0.17
Working Distance: 6.9 mm
Thread: M25
Tube Length: infinity
Objective ID: CMCB 76 20x/0.4 Leica
Order Number: 11506242

40x/0.6 HC PL FL L, Air, DIC, Leica

Immersion: Air
Collar: coverslip correction
Coverslip Thickness: 0.17
Working Distance: 3.3 - 1.9 mm
Thread: M25
Tube Length: infinity
Objective ID: CMCB 77 40x/0.6 Leica
Order Number: 11506201
Condenser

BF, DIC I, DIC II
NA 0.55, working distance 28 mm, motorized

Fluorescence Filter Cubes

motorized revolver

DAPI (set A)

single cube for DAPI, Alexa 350

  • BP 360/40 (BP 340-380), excitation filter, bandpass, Leica
  • LP 400, dichromatic mirror, longpass, Leica
  • LP 425, emission filter, longpass, Leica

Leica, set A

GFP (set F46-002)

single cube for Alexa488/GFP, FITC

  • BP 470/40 (BP 450-490), excitation filter, bandpass, Chroma
  • LP 495, dichromatic mirror, longpass, Chroma
  • BP 525/50 (BP 500-550), emission filter, bandpass, Chroma

Leica, set F46-002

Cy3 (set F46-004)

single cube for Alexa555/Cy3/RFP, TRITC

  • BP 545/25 (BP 533-558), excitation filter, bandpass, AHF
  • LP 565, dichromatic mirror, longpass, AHF
  • BP 605/70 (BP 570-640), emission filter, bandpass, AHF

Leica, set F46-004

CFP (set F46-001)

single cube for CFP

  • BP 436/20 (BP 426-446), excitation filter, bandpass, AHF
  • LP 455, dichromatic mirror, longpass, AHF
  • BP 480/40 (BP 460-500), emission filter, bandpass, AHF

Leica, set F46-001

YFP (set F46-001)

single cube for YFP

  • BP 490/20 (BP 480-500), excitation filter, bandpass, Leica
  • LP 515, dichromatic mirror, longpass, Leica
  • BP 535/30 (BP 520-550), emission filter, bandpass, Leica

Leica

polarizer (set Analysator)

polarizer cube for DIC/Polarization

Leica, set Analysator

Transmitted Illumination

Lamp (Halogen, 100W)

Fluorescence Illumination

HXP 120C

Leica, Metal halide lamp, 120W, integrated
Camera

Leica DFC 350 FX, Monochrome CCD camera, dexel size 6.45 µm

magnification 1x
bit depth 12 bit
sensor size 9 x 6.7 mm
maximum frame size 1392 x 1040 dexel
maximum full chip frame rate 21.07 - 10.54 fps
Serial number 190975006
XY Stage

manual stage

Leica, fixed stage

Computer

DELL Precision WorkStation 390

Windows 7 Professional (64 bit) operating system

  • 4 GB RAM
  • Intel Core Quad Q6700 CPU
  • NVIDIA QUADRO FX1700 grafic card
  • 2 x 19" display
  • LEICA-INV-CRTD
Software

Leica LAS AF, V 4.0.0

Additional Information
  • Inventory Number 72003
  • Contact for Service Hr. Michalski (0172/3902389)