Biopolis Dresden Imaging Platform

WF Leica upright CRTD (Leica)

Our Leica videomicroscope upright is designed for routine work on fixed sample. Both fluorescent samples and stained histological samples can be imaged due to the color acquisition function of the camera.

Category

Widefield

Location

CRTD, 2nd floor, LMF CMCB, room 2.231

Suitable for Specimen
Fixed samples
Tissue sections
Microscope Stand

Leica DM 5000, upright stand
manual objective revolver, motorized z-focus

Objectives

5x/0.15 HC PL Fluotar, Air, Leica

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 13.7 mm
Thread: M25
Tube Length: infinity
Objective ID: CMCB 22 5x/0.15 Leica
Order Number: 11506224

63x/1.2 HC PL APO lambda blue, Water, DIC, Leica

Immersion: Water
Collar: coverslip correction
Coverslip Thickness: 0.17
Working Distance: 0.22 mm
Nomarski Prism: D
Thread: M25
Tube Length: infinity
Objective ID: CMCB 10 63x/1.2 Water Leica corr.
Order Number: 11506213

20x/0.7 HC PL APO CS, Air, DIC, Leica

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 0.59 mm
Nomarski Prism: C
Thread: M25
Tube Length: infinity
Objective ID: CMCB 02 20x/0.7 Leica
Order Number: 11506513
Condenser

BF, DIC I, DIC II
NA 0.9, working distance 1.2 mm, motorized

Fluorescence Filter Cubes

motorized revolver

DAPI (set A)

single cube for DAPI, Alexa 350

  • BP 360/40 (BP 340-380), excitation filter, bandpass, Leica
  • LP 400, dichromatic mirror, longpass, Leica
  • LP 425, emission filter, longpass, Leica

Leica, set A

GFP (set L5)

single cube for Alexa488/GFP, FITC

  • BP 480/40 (BP 460-500), excitation filter, bandpass, Leica
  • LP 505, dichromatic mirror, longpass, Leica
  • BP 527/30 (BP 512-542), emission filter, bandpass, Leica

Leica, set L5

Cy3 (set N2.1)

single cube for Alexa555/Cy3/RFP, TRITC

  • BP 537/45 (BP 515-560), excitation filter, bandpass, Leica
  • LP 585, dichromatic mirror, longpass, Leica
  • LP 590, emission filter, quadro bandpass, Leica

Leica, set N2.1

polarizer (set Analysator)

polarizer cube for DIC/Polarization

Leica, set Analysator

Transmitted Illumination

Lamp (Halogen, 100W)

Fluorescence Illumination

HXP 120C

Leica, Metal halide lamp, 120W, integrated
Camera

Leica DFC 420c, Color CCD camera, dexel size 2.78 µm

port top port
bit depth 12 bit
sensor size 8.10 x 6.64 mm
maximum frame size 2592 x 1944 dexel
maximum full chip frame rate 5.9 fps
XY Stage

manual stage

Leica stage-BRC 7625, 110° Drehung, rechts

Computer

FUJITSU SIEMENS CELSIUS R650

Windows 7 Professional (32 bit) operating system

  • 2 x 2 GB RAM
  • Intel Xeon E5430 CPU
  • 19" display
  • LMF-LEICA-UPR
Software

Leica LAS AF,

Additional Information
  • Inventory Number 72002
  • Contact for Service Hr. Michalski (0172/3902389)
  • DRESDEN concept ID 1530