WF Slide scanner Axioscan CRTD (Zeiss)
It is a Zeiss widefield System dedicated to screening of fixed samples on slides. It provides automated imaging in fluorescent and transmitted light and can process up to 100 slides in one cycle.
Category
Widefield, Slide scanner
Location
CRTD, ground floor south, LMF CMCB, room -1.120
Suitable for Specimen
Suitable for Technique
Microscope Stand
Objectives
5x/0.25 Fluar, Air, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 12.5 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 177 5x/0.25 Zeiss |
| Order Number: | 420130-9900-000 |
10x/0.45 Plan-Apochromat, Air, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 2 mm |
| Nomarski Prism: | Planapo 10/0,45 II (1045-073) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 178 10x/0.45 Zeiss |
| Order Number: | 420640-9900-000 |
20x/0.8 Plan-Apochromat, Air, DIC, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.55 mm |
| Nomarski Prism: | EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 179 20x/0.8 Zeiss |
| Order Number: | 420650-9901-000 |
40x/0.95 Plan-Apochromat, Air, DIC, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.25 mm |
| Nomarski Prism: | EC PN and Fluar 40/1,3 III; PA 40/0,95 III (426944) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 180 40x/0.95 Zeiss |
| Order Number: | 420660-9970-000 |
20x/0.5 EC Plan-Neofluar, Air, DIC, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 2 mm |
| Nomarski Prism: | EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 201 20x/0.5 Zeiss |
| Order Number: | 420350-9900-000 |
| Inventory Number: | 25177348 |
Condenser
Fluorescence Filter Cubes
motorized revolver
DAPI (set 49)
single cube for Alexa 350, DAPI
- BP 387/11 (BP 381-392), excitation filter, bandpass, Zeiss
- LP 395, dichromatic mirror, longpass, Zeiss
- BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss
Zeiss, set 49
GFP (set 38 HE)
single cube for Alexa488/GFP, FITC
- LP 495, dichromatic mirror, longpass, Chroma
- BP 525/50 (BP 500-550), emission filter, bandpass, Chroma
Zeiss, set 38 HE
Cy3 (set 43 HE)
single cube for Alexa555/Cy3/RFP, TRITC
- LP 570, dichromatic mirror, longpass, Zeiss
- BP 605/70 (BP 570-640), emission filter, bandpass, Zeiss
Zeiss, set 43 HE
RFP (set 64 HE)
single cube for Alexa555/Cy3/RFP, TRITC
- LP 605, dichromatic mirror, longpass, Zeiss
- BP 647/70 (BP 612-682), emission filter, bandpass, Zeiss
Zeiss, set 64 HE
Cy5 (set 50)
single cube for Alexa633/Cy5
- LP 660, dichromatic mirror, longpass, Zeiss
- BP 690/50 (BP 665-715), emission filter, bandpass, Zeiss
Zeiss, set 50
Additional Filters
DAPI, GFP, Cy3, RFP, Cy5
- BP 387/11 (BP 381-392), excitation filter, bandpass, Zeiss
- BP 470/40 (BP 450-490), excitation filter, bandpass, Chroma
- BP 550/25 (BP 525-575), excitation filter, bandpass, Zeiss
- BP 587/25 (BP 575-600), excitation filter, bandpass, Zeiss
- BP 640/30 (BP 625-655), excitation filter, bandpass, Zeiss
Transmitted Illumination
LED (LED)
Fluorescence Illumination
HXP 120C
Zeiss, Metal halide lamp, 120W, integrated
Camera
Hitachi HV-F202SC, Color CCD camera, dexel size 4.4 µm
| port | top port |
| magnification | 1x |
| bit depth | 24 bit |
| sensor size | 7.176 x 5.319 mm |
| maximum frame size | 1600 x 1200 dexel |
| maximum full chip frame rate | 30 fps |
Hamamatsu Orca Flash 4.0 V2, Monochrome sCMOS camera, dexel size 6.5 µm
| port | top port |
| magnification | 1x |
| bit depth | 16 bit |
| sensor size | 13.3 x 13.3 mm |
| maximum frame size | 2048 x 2048 dexel |
| maximum full chip frame rate | 100 fps |
XY Stage
Computer
Software
Additional Information