Biopolis Dresden Imaging Platform

CZ6 - Zeiss LSM 880 Airy inverted (Zeiss)

Zeiss LSM 880 inverted single photon point scanning confocal system with Quasar detector (32 spectral detection channels in the GaAsP detector plus 2 PMTs) and transmitted light detector. Airy scan detector for high resolution imaging. Motorized stage for multi-point acquisition. 

Category

Laser scanning confocal

Location

MPI-CBG, LMF MPI-CBG, room 038A

Suitable for Specimen
Live samples
Fixed samples
Tissue sections
Suitable for Technique
3D imaging
Large area tile/mosaic scans
Photo manipulation (PA/FRAP/FLIP/FLAP/...)
Microscope Stand

Zeiss Axio Observer.Z1, inverted stand
motorized objective revolver, motorized z-focus

Additional z-focus device: Prior NanoScanZ

Objectives

5x/ APO, Air, Zeiss

Immersion: Air
Thread: M27
Objective ID: MPI-CBG 317 5x Zeiss
Order Number: 420639-9000-700

10x/0.45 Plan-Apochromat, Air, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 2 mm
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 318 10x/0.45 Zeiss
Order Number: 420640-9900

20x/0.8 Plan-Apochromat, Air, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 0.55 mm
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 319 20x/0.8 Zeiss
Order Number: 420650-9901

25x/0.8 LD LCI Plan-Apochromat, W / Si / Glyc / Oil, DIC, Zeiss

Immersion: W / Si / Glyc / Oil
Collar: immersion correction
Coverslip Thickness: 0-0.17
Working Distance: 0.57 mm at cover glass 0.17 mm
Nomarski Prism: LCI PN 25/0,8 II (426947)
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 320 25x/0.8 Zeiss
Order Number: 420852-9871

40x/1.2 C-Apochromat, Water, DIC, Zeiss

Immersion: Water
Collar: coverslip correction
Coverslip Thickness: 0.14-0.19
Working Distance: 0.28 mm at cover glass 0.17 mm
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 321 40x/1.2 Zeiss
Order Number: 421767-9971-711

40x/1.3 Plan-Neofluar, Oil, DIC, Zeiss

Immersion: Oil
Coverslip Thickness: 0.17
Working Distance: 0.2 mm
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MPI-CBG 24 40x/1.3 Zeiss
Order Number: 1022-818
Inventory Number: 4014010000

63x/1.3 LCI Plan-Neofluar, W / Glyc, DIC, Zeiss

Immersion: W / Glyc
Collar: immersion correction
Coverslip Thickness: 0.15-0.19
Working Distance: 0.17 mm at cover glass 0.17 mm
Nomarski Prism: LCI PN 63/1,30 II HC (426958), LCI PN 63/1,30 III (426959)
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 337 63x/1.3 Zeiss
Order Number: 420882-9970-720

63x/1.4 Plan-Apochromat, Oil, DIC, Zeiss

Immersion: Oil
Coverslip Thickness: 0.17
Working Distance: 0.19 mm
Nomarski Prism: PA 63/1,4 HC II (426952), PA 63/1,40 III (426957)
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 322 63x/1.4 Zeiss
Order Number: 420782-9900-799
Condenser

BF, DIC II, DIC III, Ph1, Ph2, Ph3
NA 0.55, working distance 26 mm, motorized

Fluorescence Filter Cubes

motorized revolver

Cy3.5 ()

single cube for Alexa 568, mCherry, Cy3.5

  • BP 560/40 (BP 540-580), excitation filter, bandpass, Zeiss
  • LP 585, dichromatic mirror, longpass, Zeiss
  • BP 630/75 (BP 593-668), emission filter, bandpass, Zeiss

DAPI (set 488049-9901-000)

single cube for DAPI

  • G 365, excitation filter, bandpass, Zeiss
  • LP 395, dichromatic mirror, longpass, Zeiss
  • BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss

, set 488049-9901-000

GFP (set 38)

single cube for Alexa488/GFP, CFP, FITC

  • BP 470/40 (BP 450-490), excitation filter, bandpass, Zeiss
  • LP 495, dichromatic mirror, longpass, Zeiss
  • BP 525/50 (BP 500-550), emission filter, bandpass, Zeiss

Zeiss, set 38

Additional Filters


  • (BP 420-460; LP 500), dichromatic mirror, longpass
  • LP 460, dichromatic mirror, longpass
  • LP 525, dichromatic mirror, longpass
  • LP 570, dichromatic mirror, longpass
  • LP 660, dichromatic mirror, longpass
  • SP 615, dichromatic mirror, shortpass


  • (BP 420-480; BP 495-620), emission filter, double bandpass
  • (BP 420-480; BP 495-550), emission filter, double bandpass
  • (BP 420-480; LP 605), emission filter, bandpass + longpass
  • (BP 465-505; LP 525), emission filter, bandpass + longpass
  • (BP 495-550; LP 570), emission filter, bandpass + longpass
  • (BP 570-620; LP 645), emission filter, bandpass + longpass
Transmitted Illumination

Lamp (Halogen, 100W)

Fluorescence Illumination

HXP 120W

Zeiss, Metal halide lamp

355nm

Laser DPSS

405nm

Laser Diode, integrated

458, 477, 488, 496, 514nm multiline

Laser Argon, integrated

561nm

Laser DPSS, integrated

594nm

Laser HeNe, integrated

633nm

Laser HeNe, integrated
XY Stage

motorized stage

Märzhauser stage for Axio Observer with

  • <link http://www.pecon.biz/wp-content/uploads/Flyer-Click-In-Premium_engl.pdf _blank external-link-new-window "Opens external link in new window">Pecon Click-In insert for 2x 35mm Petri Dishes</link>
  • <link http://www.pecon.biz/wp-content/uploads/Flyer-Click-In-Premium_engl.pdf _blank external-link-new-window "Opens external link in new window">Pecon Click-In insert for slides<br /></link>
  • heating insert P (Pecon)
  • heating insert M (Pecon)
Scan Head

LSM 880 AiryScan

conventional scanner

Internal detectors:

  • 2 confocal PMT (spectral)
  • 32 channel spectral GaAsP (Gallium Arsenide Phosphide)
  • AiryScan
  • T-PMT
Incubation

cage incubator

Zeiss

Feature:

  • Heating

  • Humidity

  • CO2

Additional Information