H2 - Nikon STORM TIRF (NIKON)
Super resolution Microscope
Nikon TIRF / N-STORM setup with two EM-CCDs for detection: at right port with a Hamamatsu W-View image splitter for simultaneous dual-color imaging; at left port with cylindrical lens for 3D-STORM; continuous laser-based autofocus PFS; strong 647nm (300mW), 561nm (150mW), and 488nm (300mW) lasers;
Suitable for N-STORM, fast dual-color TIRF, long-term focus-stabilized low-light level imaging.
Category
Widefield, TIRF
Location
MPI-CBG, LMF MPI-CBG, room 036
Suitable for Technique
Microscope Stand
Objectives
10x/0.45 Plan Fluor, Air, DIC, NIKON
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 4 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 57 10x/0.45 Nikon |
| Order Number: | MRD00101 |
20x/0.75 Plan Fluor, W / Glyc / Oil, DIC, NIKON
| Immersion: | W / Glyc / Oil |
| Collar: | immersion correction |
| Coverslip Thickness: | - |
| Working Distance: | 0.35 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 63 20x/0.75 Nikon |
| Order Number: | MRH07241 |
60x/1.2 Plan Apochromat VC, Water, DIC, NIKON
| Immersion: | Water |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.15-0.18 |
| Working Distance: | 0.22 mm |
| Nomarski Prism: | 60x II, 60x II IR |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 58 60x/1.2 Nikon |
| Order Number: | MRD07602 |
60x/1.49 Apochromat, Oil, DIC, NIKON
| Immersion: | Oil |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.13-0.21 |
| Working Distance: | 0.22 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 59 60x/1.49 Nikon |
100x/1.4 Plan Apochromat VC, Oil, DIC, NIKON
| Immersion: | Oil |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.13 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 60 100x/1.4 Nikon |
| Order Number: | MRD01901 |
100x/1.49 SR Apochromat, Oil, DIC, NIKON
| Immersion: | Oil |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.13-0.20 |
| Working Distance: | 0.12 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 61 100x/1.49 Nikon |
| Order Number: | MRY10059 |
Condenser
Fluorescence Filter Cubes
motorized revolver
TIRF 405/488/561/640 ()
quad cube
- BP 405/5; 489/4; 560/4; 637/14 (BP 400-410; 485-493; 555-564; 630-644), dichromatic mirror, quadro bandpass, Chroma
AHF
TIRF 405/488/561/640 + Emission filters ()
quad cube
- BP 405/5; 489/4; 560/4; 637/14 (BP 400-410; 485-493; 555-564; 630-644), dichromatic mirror, quadro bandpass, Chroma
- BP 446/34, 523/42, 600/36, 677/28 (BP 430-462;502-544; 582-617; 663-690), emission filter, quadro bandpass, Semrock
AHF
YFP ()
single cube for YFP
- BP 500/24 (BP 488-512), excitation filter, bandpass, Semrock
- LP 520, dichromatic mirror, longpass, Semrock
- BP 542/27 (BP 528-555), emission filter, bandpass, Semrock
AHF
mCherry ()
single cube for Alexa 568, Alexa 555
- BP 562/40, excitation filter, bandpass, Semrock
- LP 593, dichromatic mirror, longpass, Semrock
- BP 624/40 (BP 604-644), emission filter, bandpass, Semrock
AHF
polarizer ()
single cube for Alexa 568, Alexa 555
AHF
EMPTY ()
empty for through position
- empty
AHF
motorized revolver
DAPI
single cube for DAPI
- BP 406/15, excitation filter, bandpass, Semrock
- LP 495, dichromatic mirror, longpass, Chroma
- BP 457/50 (BP 432-482), emission filter, bandpass, Semrock
AHF
eGFP
single cube for DAPI
- BP 470/40 (BP 450-490), excitation filter, bandpass, Chroma
- LP 660, dichromatic mirror, longpass, Chroma
- BP 525/50, emission filter, bandpass, Chroma
AHF
Universal Green/Red
double cube for Alexa488/GFP, mCherry
- BP 470/40, excitation filter, bandpass, Chroma
- BP ~525/40, ~632/55 (BP 505-545; 605-660), dichromatic mirror, double bandpass, Chroma
- BP 522/41; BP 632/62 (BP 501-542; BP 601-663), emission filter, double bandpass, Chroma
AHF
eGFP narrow
single cube for Alexa488/GFP
- BP 510/40 (BP 490-530), emission filter, bandpass
AHF
Cy5
single cube for Alexa488/GFP
- BP 620/60 (BP 590-650), excitation filter, bandpass, Chroma
- LP 660, dichromatic mirror, longpass, Chroma
- BP 700/75, emission filter, bandpass, Chroma
AHF
EMPTY
empty for through position
- empty
AHF
Additional Filters
Alexa 568, Alexa 594, Alexa 594, YFP, Alexa 568
- BP 617/73, emission filter, bandpass, Semrock
- LP 488 Razor edge, dichromatic mirror, longpass, Semrock
- LP 664 - RazorEdge, dichromatic mirror, longpass, Semrock
- BP 447/60 (BP 417-477), emission filter, bandpass, AHF
- empty
- BP 512/23; BP630/91 (BP 501-524; BP 585-676), emission filter, double bandpass, Semrock
- BP 628/40 (BP 608-648), emission filter, bandpass, Semrock
- BP 525/30 (BP 510-540), emission filter, bandpass, Semrock
- BP 607/36 (BP 589-625), emission filter, bandpass, Semrock
- BP 520/15 (BP 512-527), emission filter, bandpass, Semrock
Alexa 594
- LP 560, dichromatic mirror, longpass, Chroma
- SP 560, dichromatic mirror, shortpass, Chroma
- BP 535/30 (BP 520-550), emission filter, bandpass, Chroma
- BP 628/40 (BP 608-648), emission filter, bandpass, Semrock
- mirror
Transmitted Illumination
(Nikon 100W Pillar Diascopic Illuminator)
Fluorescence Illumination
405nm
Laser Diode, integrated
488nm
Laser DPSS, integrated
561nm
Laser DPSS, integrated
640nm
Laser DPSS
CoolLED pE-2 400/470/565/635
CoolLED, LED, integrated
Metal Halide Lamp
NIKON, Metal halide lamp, 130 W, integrated
TIRF Illuminator 405/488/561/640
NIKON, Laser, integrated
Camera
Andor iXON 897 EMCCD, Monochrome EMCCD camera, dexel size 16 µm
| port | Left side port |
| bit depth | 16 bit |
| sensor size | 8.2 x 8.2 mm |
| maximum frame size | 512 x 512 dexel |
| maximum full chip frame rate | 56-11.074 fps |
Andor iXon Ultra 897, Monochrome EMCCD camera, dexel size 16 µm
| port | Right side port |
| bit depth | 16 bit |
| sensor size | 8.2 x 8.2 mm |
| maximum frame size | 512 x 512 dexel |
| maximum full chip frame rate | 56-11.074 fps |
XY Stage
Hardware Autofocus
NIKON Perfect Focus System
Incubation
Additional Information