Biopolis Dresden Imaging Platform

H3 - Andor STORM Spinning disc (Andor)

Real-Time Confocal Microscope

Fast, "real-time" confocal imaging with FRAPPA capability. Illumination equipment: TM-LED, Andor AMH, 405nm diode laser + 488 nm solid-state laser Coherent Sapphire + 561 nm solid-state laser + 640 nm solid-state laser.

Suitable for confocal imaging, photobleaching and -activation of DAPI,EGFP, mCherry, Cy5 with high temporal and spatial resolution.

 

Link to pixel sizes

Category

Spinning disk

Location

MPI-CBG, LMF MPI-CBG, room 340

Suitable for Technique
Superresolution Localisation Microscopy (PALM/STORM/...)
Microscope Stand

Andor Eclipse Ti, inverted stand
motorized objective revolver

Additional magnification: Optovar 1.5x

Objectives

10x/0.45 Plan Fluor, Air, NIKON

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 4 mm
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 62 10x/0.45 Nikon

40x/0.95 Plan Apochromat, Air, DIC, NIKON

Immersion: Air
Collar: coverslip correction
Coverslip Thickness: 0.11-0.23
Working Distance: 0.25 – 0.17 mm
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 328 40x/0.95 Nikon
Order Number: MRD00405

60x/1.2 Plan Apochromat VC, Water, DIC, NIKON

Immersion: Water
Collar: coverslip correction
Coverslip Thickness: 0.15-0.18
Working Distance: 0.27 mm
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 243 60x/1.2 Nikon
Order Number: MRD07602

100x/1.45 Plan Apochromat, Oil, DIC, NIKON

Immersion: Oil
Coverslip Thickness: 0.17
Working Distance: 0.13 mm
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 64 100x/1.45 Nikon

100x/1.45 Plan Apochromat, Oil, DIC, NIKON

Immersion: Oil
Coverslip Thickness: 0.17
Working Distance: 0.13 mm
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 244 100x/1.45 Nikon
Order Number: MRD01905
Condenser

BF, DIC, Ph0
NA 0.52, working distance 30 mm

Transmitted Illumination

LED (LED)

Fluorescence Illumination

405nm

Laser Diode, integrated

488nm

Laser DPSS, integrated

561nm

Laser DPSS, integrated

640nm

Laser DPSS

AMD-200-FOS

Andor, Metal halide lamp, 200 mW, integrated
Camera

Andor iXON 897 EMCCD, Monochrome EMCCD camera, dexel size 16 µm

port left side port
bit depth 16 bit
sensor size 8.2 x 8.2 mm
maximum frame size 512 x 512 dexel
maximum full chip frame rate 56 fps
Scan Head

Yokogawa, CSU-X1

  • max. spinning disk rotation speed: 1500 - 10.000 rpm
  • physical pinhole diameter: 25 µm
  • physical pinhole spacing: 250 µm
Additional Information