Biopolis Dresden Imaging Platform

L2 - Long Term Timelapse II (Andor)

Wide-Field Microscope

Motorized inverted wide-field system.

Suitable for long-term imaging of living cells in glass-bottom-dishes or -plates.

Category

Widefield

Location

MPI-CBG, LMF MPI-CBG, room 240

Suitable for Specimen
protein droplets
Suitable for Technique
Multiple positions
Timelapse imaging
Microscope Stand

Andor Axiovert 200M, inverted stand
motorized objective revolver, motorized z-focus

Objectives

5x/0.15 Plan-Neofluar, Air, Ph1, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 13.6 mm
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MPI-CBG 73 5x/0.15 Zeiss
Order Number: 440782-9902
Inventory Number: 4051910000

10x/0.3 Plan-Neofluar, Air, Ph1, Zeiss

Immersion: Air
Coverslip Thickness: -
Working Distance: 5.2 mm
Nomarski Prism: EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940)
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MPI-CBG 74 10x/0.3 Zeiss
Order Number: 440331
Inventory Number: 4024530000

20x/0.4 LD A-Plan, Air, Ph2, Zeiss

Immersion: Air
Collar: coverslip correction
Coverslip Thickness: 0-1.5
Working Distance: 10.2 mm at cover glass 1.0 mm
Nomarski Prism: LD 20x/0,40 epi+ (444447)
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MPI-CBG 75 20x/0.4 Zeiss
Order Number: 440845
Inventory Number: 4007120000

25x/0.8 Plan-Neofluar, W / Glyc / Oil, Ph2, Zeiss

Immersion: W / Glyc / Oil
Collar: immersion correction
Coverslip Thickness: -
Working Distance: 0.21 mm
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MPI-CBG 76 25x/0.8 Zeiss
Order Number: 440545

40x/0.75 Plan-Neofluar, Air, Ph2, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 0.71 mm
Nomarski Prism: EC PN 40/0,75 II; 100/1,30III (426945)
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MPI-CBG 77 40x/0.75 Zeiss
Order Number: 440351
Inventory Number: 4078140000

40x/1.2 C-Apochromat, Water, Ph2, Zeiss

Immersion: Water
Collar: coverslip correction
Coverslip Thickness: 0.14-0.19
Working Distance: 0.28 mm at cover glass 0.17 mm
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MPI-CBG 78 40x/1.2 Zeiss
Order Number: 441757-9970

63x/0.75 LD A-Plan, Air, Ph2, Zeiss

Immersion: Air
Collar: coverslip correction
Coverslip Thickness: 0-1.5
Working Distance: 1.5 mm at cover glass 1.0 mm
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MPI-CBG 79 63x/0.75 Zeiss
Order Number: 440861

100x/1.4 Plan-Apochromat, Oil, DIC, Zeiss

Immersion: Oil
Coverslip Thickness: 0.17
Working Distance: 0.17 mm
Nomarski Prism: PA 100/1,4 HC II (426949), PA 100/1,40 III (426941)
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MPI-CBG 80 100x/1.4 Zeiss
Order Number: 440782
Inventory Number: 4034680000

100x/1.3 Plan-Neofluar, Oil, Ph3, Zeiss

Immersion: Oil
Coverslip Thickness: 0.17
Working Distance: 0.2 mm
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MPI-CBG 212 100x/1.3 Zeiss
Order Number: 1031-171
Condenser

NA 0.55, working distance 26 mm, motorized

Transmitted Illumination

LED (LED)

Fluorescence Illumination

CoolLED pE-4000

CoolLED, LED, integrated
Camera

Andor Zyla PLUS sCMOS, Monochrome sCMOS camera, dexel size 6.5 µm

port left port
bit depth 12 bit
sensor size 13.3 x 13.3 mm
maximum frame size 2048 x 2048 dexel
Inventory number MOZG04103210000

PCO Dimax S4, Monochrome sCMOS camera, dexel size 11 µm

port top port
bit depth 12 bit
sensor size 22.18 x 22.18 mm
maximum frame size 2016 x 2016 dexel
maximum full chip frame rate 1279 fps
Inventory number MOZG04096900000
Serial number 9000000436
XY Stage

motorized stage

Additional Information