SD1 - Andor Spinning Disc (Andor)
Fast, "real-time" confocal imaging. The microscope is equipped with three lasers for imaging 488 nm, 561 nm and 640 nm, a laser for laser ablatin and two cameras and sCMOS (for higher resolution) and an EMCCD (for sensitivity).
The set-up is suitable for confocal imaging of EGFP, mCherry, dTomato, Draq5 and the likes with high temporal and spatial resolution.
Link to the pixel sizes
Category
Spinning disk
Location
MPI-CBG, LMF MPI-CBG, room 038A
Suitable for Specimen
Suitable for Technique
Microscope Stand
Objectives
10x/0.4 U Plan SApo, Air, OLYMPUS
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 3.1 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 186 10x/0.4 Olympus |
| Order Number: | UPLSAPO10X2 |
| Inventory Number: | 4077980000 |
20x/0.5 U Plan Fl, Air, OLYMPUS
| Coverslip Thickness: | 0.17 |
| Working Distance: | 2.1 mm |
| Nomarski Prism: | A |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 141 20x/0.5 Olympus |
| Order Number: | UPLFLN20X |
40x/0.75 U Plan FL N, Air, Ph2, OLYMPUS
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.51 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 188 40x/0.75 Olympus |
| Order Number: | UPLFLN40XPH |
| Inventory Number: | 4095670000 |
40x/0.9 U Plan SApo, Air, Ph2, OLYMPUS
| Immersion: | Air |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.11-0.23 |
| Working Distance: | 0.18 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 151 40x/0.9 Olympus |
| Order Number: | UPLSAPO40X |
40x/1.15 U Apo, Water, Ph2, OLYMPUS
| Immersion: | Water |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.13-0.25 |
| Working Distance: | 0.25 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 187 40x/1.15 Olympus |
| Order Number: | UAPON40XW340 |
| Inventory Number: | 4090380000 |
60x/1.2 U Plan SApo, Water, Ph2, OLYMPUS
| Immersion: | Water |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.13-0.21 |
| Working Distance: | 0.28 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 190 60x/1.2 Olympus |
| Order Number: | UPLSAPO60XW |
| Inventory Number: | 4075200000 |
60x/0.65 U Plan Fl, Oil, Ph3, OLYMPUS
| Immersion: | Oil |
| Collar: | NA correction |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.12 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 191 60x/1.25-0.65 Olympus |
| Order Number: | UPLFLN60XOIPH |
100x/1.4 U Plan SApo, Oil, Ph3, OLYMPUS
| Immersion: | Oil |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.13 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 193 100x/1.4 Olympus |
| Order Number: | UPLSAPO100XO |
| Inventory Number: | 4068520000 |
Condenser
Fluorescence Filter Cubes
manual revolver
DAPI ()
single cube for DAPI, Alexa 405
- BP 377/50 (BP 352-402), excitation filter, bandpass, AHF
- LP 409, dichromatic mirror, longpass, AHF
- BP 447/60 (BP 417-477), emission filter, bandpass, AHF
OLYMPUS
eGFP ()
single cube for Alexa488/GFP
OLYMPUS
RFP ()
single cube for Alexa 568
OLYMPUS
Laser ablation ()
single cube for Laser manipulation
- LP 405, dichromatic mirror
OLYMPUS
EMPTY ()
empty for through position
- empty
OLYMPUS
polarizer ()
polarizer cube for through position
OLYMPUS
Additional Filters
YFP, Cy5, CFP, YFP, Cy5, Alexa 647, TRITC, Cy5, Alexa 488, eGFP, Alexa 568, Alexa 555, Alexa 647, Cy5
- BP 525/30 (BP 510-540), emission filter, bandpass
- BP 624/40 (BP 604-644), emission filter, bandpass
- BP 445/40 (BP 425-465), emission filter, bandpass
- BP 512/23; BP630/91 (BP 497-558, 612-693), emission filter, double bandpass
- BP 525/30; 650/? (BP 510-540; BP 650/?), emission filter, double bandpass
- BP 605/70 (BP 570-640), emission filter, bandpass
- LP 488 Razor edge, emission filter, longpass
- LP 568 Razor edge, emission filter, longpass
- LP 635, emission filter, longpass
Transmitted Illumination
Lamp (Halogen, 100W)
Fluorescence Illumination
HBO
Mercury arc lamp, 100W
488nm
Laser DPSS, integrated
561nm
Laser DPSS, integrated
640nm
Laser DPSS
Camera
Andor iXon Ultra 897, Monochrome EMCCD camera, dexel size 16 µm
| port | left side port |
| bit depth | 14 bit |
| sensor size | 8.2 x 8.2 mm |
| maximum frame size | 512 x 512 dexel |
| maximum full chip frame rate | 35-549 fps |
Andor Neo sCMOS, Monochrome sCMOS camera, dexel size 6.5 µm
| port | right side port |
| bit depth | 16 bit |
| sensor size | 16.6 x 14.0 mm |
| maximum frame size | 2560 x 2160 dexel |
| maximum full chip frame rate | 30 fps |
XY Stage
Scan Head
Incubation
Additional Equipment
Micropoint - 405nm laser for ablation
Additional Information