Biopolis Dresden Imaging Platform

W3 - Zeiss CCD Upright !! Retired !! (Zeiss)

It is an upright, motorized microscope with medium sensitivity CCD camera. For quick checking of your samples.

Category

Widefield

Location

MPI-CBG, LMF MPI-CBG, room U32

Suitable for Specimen
Fixed samples
Tissue sections
Organoids
Microscope Stand

Zeiss Axioplan 2, upright stand
motorized objective revolver, motorized z-focus

Objectives

5x/0.25 Fluar, Air, Zeiss

Immersion: Air
Coverslip Thickness: -
Working Distance: 12.5 mm
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MPI-CBG 251 5x/0.25 Zeiss
Order Number: 440125

10x/0.3 Plan-Neofluar, Air, Zeiss

Immersion: Air
Collar: coverslip correction
Coverslip Thickness: 0.17
Working Distance: 5.2 mm
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MPI-CBG 228 10x/0.3 Zeiss
Order Number: 440330

20x/0.5 Achroplan, Water, Ph2, Zeiss

Immersion: Water
Working Distance: 1.9 mm
Nomarski Prism: 20/0,5W II (444448)
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MPI-CBG 140 20x/0.5 Zeiss
Order Number: 440049

40x/0.75 Plan-Neofluar, Air, Ph2, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 0.71 mm
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MPI-CBG 88 40x/0.75 Zeiss
Order Number: 440350

63x/0.8 Achroplan, Air, Ph2, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 0.29 mm
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MPI-CBG 229 63x/0.8 Zeiss
Order Number: 440060

63x/1.2 C-Apochromat, Water, Ph2, Zeiss

Immersion: Water
Collar: coverslip correction
Coverslip Thickness: 0.15-0.17
Working Distance: 0.24 mm
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MPI-CBG 255 63x/1.2 Zeiss
Order Number: 440668

63x/1.4 Plan-Apochromat, Oil, DIC, Zeiss

Immersion: Oil
Coverslip Thickness: 0.17
Working Distance: 0.19 mm
Nomarski Prism: PA 63/1,4 HC II (426952), PA 63/1,40 III (426957)
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MPI-CBG 170 63x/1.4 Zeiss
Order Number: 440762-9904

100x/1.3 Plan-Neofluar, Oil, DF, Zeiss

Immersion: Oil
Collar: NA correction
Coverslip Thickness: 0.17
Working Distance: 0.2 mm
Thread: M27
Tube Length: infinity
Objective ID: MPI-CBG 252 100x/1.3-0.7 Zeiss
Order Number: 420496-9900

100x/1.4 Plan-Apochromat, Oil, DIC, Zeiss

Immersion: Oil
Coverslip Thickness: 0.17
Working Distance: 0.17 mm
Nomarski Prism: PA 100/1,4 HC II (426949), PA 100/1,40 III (426941)
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MPI-CBG 103 100x/1.4 Zeiss
Order Number: 440782-9902
Condenser

H/DIC, Ph1, Ph2, Ph3, DF, DIC II, DIC III
NA 0.9, working distance 1.2 mm, manual

Fluorescence Filter Cubes

motorized revolver

DAPI ()

single cube for DAPI, Alexa 350

  • BP 390/18 (BP 381-399), excitation filter, bandpass, AHF
  • LP 416, dichromatic mirror, longpass, AHF
  • BP 460/60 (BP 430-490), emission filter, bandpass, AHF

CFP ()

single cube for CFP

  • BP 434/17 (BP 425-443), excitation filter, bandpass, AHF
  • LP 452, dichromatic mirror, longpass, AHF
  • BP 479/40 (BP 459-499), emission filter, bandpass, AHF

TRITC ()

single cube for TRITC, Alexa 555, Cy3

  • BP 543/22 (BP 532-554), excitation filter, bandpass, AHF
  • LP 562, dichromatic mirror, longpass, AHF
  • BP 593/40 (BP 573-613), emission filter, bandpass, AHF

mCherry ()

single cube for mCherry, Cy3.5

  • BP 562/40 (BP 542-582), excitation filter, bandpass, AHF
  • LP 593, dichromatic mirror, longpass, AHF
  • BP 641/75 (BP 604-679), emission filter, bandpass, AHF

Cy5 ()

single cube for Alexa633/Cy5

  • BP 628/40 (BP 608-648), excitation filter, bandpass, AHF
  • LP 660, dichromatic mirror, longpass, AHF
  • BP 692/40 (BP 672-712), emission filter, bandpass, AHF

eGFP ()

single cube for Alexa488/GFP, FITC

  • BP 472/30 (BP 457-487), excitation filter, bandpass, AHF
  • LP 495, dichromatic mirror, longpass, AHF
  • BP 520/35 (BP 503-538), emission filter, bandpass, AHF

YFP ()

single cube for YFP

  • BP 500/20 (BP 480-520), excitation filter, bandpass, AHF
  • LP 515, dichromatic mirror, longpass, AHF
  • BP 535/30 (BP 520-550), emission filter, bandpass, AHF

Transmitted Illumination

Lamp (Halogen, 100W)

Fluorescence Illumination

X-Cite 120W

X-Cite, Metal halide lamp
Camera

Imagine Source DMK 31AF03, Monochrome CCD camera, dexel size 4.65 µm

port top port
bit depth 8 bit
sensor size 1/3 inch
maximum frame size 1024x768 dexel
maximum full chip frame rate 30 fps

Diagnostic Instrument RT Spot, Monochrome CCD camera, dexel size 6.7 µm

port top port
magnification 1x
bit depth 12 bit
maximum frame size 1600 x 1200 dexel
XY Stage

manual stage

manual xy stage with

  • holder for 1 slide
Additional Equipment

reflection darkfield oil condenser

Additional Information