Biopolis Dresden Imaging Platform

CFCI-SD 01 Spinning Disk (OLYMPUS)

The Olympus spinning disc microscope is designed for fast "real-time" confocal imaging of living samples. Furthermore that system is especially designed for mouse intravital microscopy. The microscope is also capable for brightfield including contrast techniques like DIC, darkfield and polarized light.

Category

Spinning disk

Location

MTZ, LMF & EMF CFCI, room A.20.028

Suitable for Specimen
Live samples
Fixed samples
Whole organisms
Whole organs
Suitable for Technique
3D imaging
Mouse intravital microscopy
Multiple positions
Large area tile/mosaic scans
Timelapse imaging
Microscope Stand

OLYMPUS IX 83, inverted stand
motorized objective revolver, motorized z-focus

Additional z-focus device: Olympus Fast 4D Imaging Piezo Z stage

Objectives

4x/0.16 U Plan SApo, Air, OLYMPUS

Immersion: Air
Coverslip Thickness: -
Working Distance: 13 mm
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MTZ O_4x_0,16_01 4x/0.16 Olympus
Order Number: UPLSAPO4X - UIS 2

10x/0.4 U Plan SApo, Air, DIC, OLYMPUS

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 3.1 mm
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MTZ O_10x_0,4_01 10x/0.4 Olympus
Order Number: UPLSAPO10X2 - UIS 2

20x/0.75 U Plan SApo, Air, DIC, OLYMPUS

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 0.6 mm
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MTZ O_20x_0,75_01 20x/0.75 Olympus
Order Number: UPLSAPO20X - UIS 2

40x/0.95 U Plan SApo, Air, DIC, OLYMPUS

Immersion: Air
Collar: coverslip correction
Coverslip Thickness: 0.11-0.23
Working Distance: 0.18 mm
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MTZ O_40x_0,95_01 40x/0.95 Olympus
Order Number: UPLSAPO40X - UIS 2

60x/1.2 U Plan SApo, Water, DIC, OLYMPUS

Immersion: Water
Collar: coverslip correction
Coverslip Thickness: 0.13-0.21
Working Distance: 0.28 mm
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MTZ O_60x_1,2_01 60x/1.2 Olympus
Order Number: UPLSAPO60XW - UIS 2

60x/1.42 Plan Apo, Oil, DIC, OLYMPUS

Immersion: Oil
Coverslip Thickness: 0.17
Working Distance: 0.15 mm
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MTZ O_60x_1.42_01 60x/1.42 Olympus
Order Number: PLAPON60XO - UIS 2

30x/1.05 U Plan SApo, Silicone, DIC, OLYMPUS

Immersion: Silicone
Collar: coverslip correction
Coverslip Thickness: 0.13-0.19
Working Distance: 0.8 mm
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MTZ O_30x_1.05_01 Olympus
Order Number: UPLSAPO30XS
Condenser

H/DIC
NA 0.55, working distance 27 mm, motorized

Fluorescence Filter Cubes

revolver

GFP ()

single cube for Alexa488/GFP

  • BP 470/20 (BP 460-480), excitation filter
  • LP 485, dichromatic mirror
  • BP 518/45 (BP 495-540), emission filter

OLYMPUS

RFP ()

single cube for Alexa 568, Cy3, RFP

  • BP 545/20 (BP 535-555), excitation filter
  • LP 565, dichromatic mirror
  • BP 598/55 (BP 570-625), emission filter

OLYMPUS

Cy5 (set 49006)

single cube for Alexa633/Cy5

  • BP 620/60 (BP 590-650), excitation filter, bandpass, OLYMPUS
  • LP 660, dichromatic mirror, longpass, OLYMPUS
  • BP 700/75 (BP 663-738), emission filter, bandpass, OLYMPUS

OLYMPUS, set 49006

Additional Filters

Alexa 405, Alexa 488, Alexa 568, Alexa 647, Alexa 488, GFP, Alexa 488, Alexa 568, GFP, RFP, Alexa 647, Cy5

Transmitted Illumination

Lamp (Halogen, 100W)

Fluorescence Illumination

HBO

Mercury arc lamp, 100W

405nm

Laser Diode, integrated

488nm

Laser DPSS, integrated

561nm

Laser DPSS, integrated

640nm

Laser Diode, integrated
Camera

Andor iXon Ultra 897, Monochrome EMCCD camera, dexel size 16 µm

port Left side port
bit depth 16 bit
sensor size 8.2 x 8.2 mm
maximum frame size 512 x 512 dexel
maximum full chip frame rate 56 fps
XY Stage

motorized stage

Prior PR-IX3-STG3 with

  • universal disholder
  • multiwell plate holder
  • low profile slide holder (75x25mm)
  • heating insert P-Set 2000 (Pecon)
  • heating insert P (Pecon)
Hardware Autofocus

OLYMPUS IX81-ZDC 2

Scan Head

Yokogawa, CSU-X1

  • max. spinning disk rotation speed: 1500 - 5000 rpm
  • maximum scanning speed: 1000 fps
  • physical pinhole diameter: 50 µm
  • physical pinhole spacing: 250 µm
Incubation

stage top incubator

P-Set 2000 for dishes and slides, Pecon

Feature:

  • CO2

  • Heating

  • Humidity

Computer

Windows 7 Professional (64 bit) operating system

  • 2 GB RAM
  • Intel Xeon E5-1620 CPU
  • NVIDIA QUADRO 2000 grafic card
  • 27" display
Additional Equipment

Setup for mouse intravital microscopy:

  • Surgery unit with heating lamp
  • Induction box for pre-anesthesia
  • BioLite Intubation System
  • Mouse Ventilation System: Minivent (Hugo Sachs)
  • Silicone heating plate with temperature sensor (Friedrich Freek)

Additional Information
  • Inventory Number 00102524/0
  • SIP Number 2K88116
  • Date of Installation 30.08.2013
  • End of Warranty 30.08.2015
  • Service Visits Upgrades 1/2015