CO1 - Olympus Fluoview 1000
Line 17: | Line 17: | ||
|obj1=Olympus UPlanSApochromat 10x 0.4 | |obj1=Olympus UPlanSApochromat 10x 0.4 | ||
|obj2=Olympus UPlanApo 40x 0.85 | |obj2=Olympus UPlanApo 40x 0.85 | ||
− | |obj3=Olympus | + | |obj3=Olympus UPlanSApochromat 20x 0.85 Oil |
|obj4=Olympus UPlanSApo 40x 1.35 Oil | |obj4=Olympus UPlanSApo 40x 1.35 Oil | ||
|obj5=Olympus UPlanSApo 60x 1.35 Oil | |obj5=Olympus UPlanSApo 60x 1.35 Oil |
Revision as of 11:14, 24 October 2014
Directions
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
microscope | Olympus - BX 61, Inverted stand, motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC | ||
objectives | |||
illumination | |||
detection | [[detection::point scanner, 4 confocal PMTs, two independent spectral detection channels, each one configured with a diffraction grating and variable slit for high-resolution wavelength separation and high-speed bandwidth selection, two filter based detection channels, T-PMT, dichromatic mirrors for excitation/emission splitting, one pinhole]] | ||
reflectors | [[reflectors::Filter Cube 1. U-MWU2. BP330-385 BA420 DM400
Filter Cube 2. U-MWB2. BP460-490 BA520IF (High performance interference filter) DM500 | ||
features | Objectives 40x 1.25 SIL and 60x 1.3 SIL are shared objectives and need to be booked separately via the LMF booking database!
Two independent and fully synchronized laser scanners for simultaneous laser stimulation and observation, laser stimulation can be performed using the imaging laser or a 405nm picosecond Diode laser (PicoQuant), sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, Kalman averaging, summarizing, scan zoom and rotation; advanced use of motorized stage due to MATL-software, which allows independent multi-point acquisition and tiling + some simple stitching"Objectives 40x 1.25 SIL and 60x 1.3 SIL are shared objectives and need to be booked separately via the LMF booking database! Two independent and fully synchronized laser scanners for simultaneous laser stimulation and observation, laser stimulation can be performed using the imaging laser or a 405nm picosecond Diode laser (PicoQuant), sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, Kalman averaging, summarizing, scan zoom and rotation; advanced use of motorized stage due to MATL-software, which allows independent multi-point acquisition and tiling + some simple stitching" cannot be used as a page name in this wiki. | ||
software | FV10-ASW 1.7 | ||
incubation | Stage incubator (T+CO2) | ||
links |
| ||
inv.nr. | MOZG0407421000 10.1.38.205 |