Advanced system check

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MPI-CBG LMF
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This section suggests protocols for the evaluation of high end light microscopes. The tests should be carried out in a regular manner. Although the protocols are set up for the use with [[confocal]] laser scanning microscopes (CLSM), most of them can be adopted for the use with wide-field systems and other types of light microscopes.
 
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# [[ASC Fluorescence and Brightfield Imaging|Fluorescence and Brightfield Imaging]]
Advanced System Check
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# [[ASC Field Illumination|Field Illumination]]
 
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# [[ASC Color Overlay|Color Overlay]]
Protocol (draft)
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# [[ASC Point Spread Function|Point Spread Function]]
 
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# [[ASC Z-Resolution|Z-Resolution]]
*fluorescence and bright field imaging (all systems)
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The individual protocols are optimized for PDF output via the "Print as PDF"-feature in the sidebar.
**purpose: test general performance of the system - basic functions work fine?
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[[category:Manuals|A]]
**requirements: Zeiss/Leica Convallaria demo sample
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[[category:ASC]]
**work flow: acquire two images in basic resolution (512x512), blue ex / green em plus transmitted light image, using 10x objective
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*psf
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**purpose:
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**requirements:
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**work flow:
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*overlay UV/V and VIS (confocal systems with separate UV/V laser coupling)
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**purpose: check the coupling precision of the UV/V laser
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**requirements: 0.2/0.5µm fluorescent beads sample
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**work flow: open pinhole(s), use high resolution apochromatic lens (NA 1.2 or above), acquire two images (UV/V plus VIS channel) with good sampling (pixel size ~100nm)
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Latest revision as of 14:04, 1 October 2009

This section suggests protocols for the evaluation of high end light microscopes. The tests should be carried out in a regular manner. Although the protocols are set up for the use with confocal laser scanning microscopes (CLSM), most of them can be adopted for the use with wide-field systems and other types of light microscopes.

  1. Fluorescence and Brightfield Imaging
  2. Field Illumination
  3. Color Overlay
  4. Point Spread Function
  5. Z-Resolution

The individual protocols are optimized for PDF output via the "Print as PDF"-feature in the sidebar.

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