CO1 - Olympus Fluoview 1000
From BioDIP
(Difference between revisions)
Line 8: | Line 8: | ||
|room=038A | |room=038A | ||
|Facility-logo=MPI-CBG LMF.jpg | |Facility-logo=MPI-CBG LMF.jpg | ||
+ | |Institute-logo=Logo MPI-CBG.jpg | ||
|description=Single photon point scanning [[Olympus FV1000]] [[confocal]] microscope with four confocal PMTs and one transmitted light detector. Inverted configuration with additional scanner and pulsed picosecond laser | |description=Single photon point scanning [[Olympus FV1000]] [[confocal]] microscope with four confocal PMTs and one transmitted light detector. Inverted configuration with additional scanner and pulsed picosecond laser | ||
|applications=ablation experiments | |applications=ablation experiments |
Revision as of 12:23, 18 November 2013
Directions
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
microscope | Olympus - BX 61, Inverted stand, motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC | ||
objectives | |||
illumination | |||
detection | [[detection::point scanner, 4 confocal PMTs, two independent spectral detection channels, each one configured with a diffraction grating and variable slit for high-resolution wavelength separation and high-speed bandwidth selection, two filter based detection channels, T-PMT, dichromatic mirrors for excitation/emission splitting, one pinhole]] | ||
reflectors | [[reflectors::Filter Cube 1. U-MWU2. BP330-385 BA420 DM400
Filter Cube 2. U-MWB2. BP460-490 BA520IF (High performance interference filter) DM500 | ||
features | Two independent and fully synchronized laser scanners for simultaneous laser stimulation and observation, laser stimulation can be performed using the imaging laser or a 405nm picosecond Diode laser (PicoQuant), sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, Kalman averaging, summarizing, scan zoom and rotation; advanced use of motorized stage due to MATL-software, which allows independent multi-point acquisition and tiling + some simple stitching"Two independent and fully synchronized laser scanners for simultaneous laser stimulation and observation, laser stimulation can be performed using the imaging laser or a 405nm picosecond Diode laser (PicoQuant), sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, Kalman averaging, summarizing, scan zoom and rotation; advanced use of motorized stage due to MATL-software, which allows independent multi-point acquisition and tiling + some simple stitching" cannot be used as a page name in this wiki. | ||
software | FV10-ASW 1.7 | ||
incubation | Stage incubator (T+CO2) | ||
links |
| ||
inv.nr. | MOZG0407421000 10.1.38.205 |