CO1 - Olympus Fluoview 1000
| Line 21: | Line 21: | ||
|obj5=Olympus UPlanSApo 60x 1.35 Oil | |obj5=Olympus UPlanSApo 60x 1.35 Oil | ||
|obj6=Olympus UPlanApo 60x 1.2 W | |obj6=Olympus UPlanApo 60x 1.2 W | ||
| + | |obj7=Olympus UPLSAPO 40x 1.25 SIL | ||
| + | |obj8=Olympus UPLSAPO 60x 1.3 SIL | ||
|ill1=Transmitted Light (Halogen) | |ill1=Transmitted Light (Halogen) | ||
|ill2=Fluorescence (Metal Halide, X-Cite, 120W) | |ill2=Fluorescence (Metal Halide, X-Cite, 120W) | ||
| Line 32: | Line 34: | ||
Filter Cube 2. [http://www.olympusamerica.com/seg_section/uis2/seg_uis2_mr_umwb2.asp U-MWB2]. BP460-490 BA520IF (High performance interference filter) DM500<br> | Filter Cube 2. [http://www.olympusamerica.com/seg_section/uis2/seg_uis2_mr_umwb2.asp U-MWB2]. BP460-490 BA520IF (High performance interference filter) DM500<br> | ||
Filter Cube 3. [http://www.olympusamerica.com/seg_section/uis2/seg_uis2_mr_umwg2.asp U-MWG2]. BP510-550 BA590 DM570 | Filter Cube 3. [http://www.olympusamerica.com/seg_section/uis2/seg_uis2_mr_umwg2.asp U-MWG2]. BP510-550 BA590 DM570 | ||
| − | |features=Two independent and fully synchronized laser scanners for simultaneous laser stimulation and observation, laser stimulation can be performed using the imaging laser or a 405nm picosecond Diode laser (PicoQuant), sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, Kalman averaging, summarizing, scan zoom and rotation; advanced use of motorized stage due to MATL-software, which allows independent multi-point acquisition and tiling + some simple stitching | + | |features=Objectives 40x 1.25 SIL and 60x 1.3 SIL are shared objectives and need to be booked separately via the LMF booking database! |
| + | Two independent and fully synchronized laser scanners for simultaneous laser stimulation and observation, laser stimulation can be performed using the imaging laser or a 405nm picosecond Diode laser (PicoQuant), sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, Kalman averaging, summarizing, scan zoom and rotation; advanced use of motorized stage due to MATL-software, which allows independent multi-point acquisition and tiling + some simple stitching | ||
|software=FV10-ASW 1.7 | |software=FV10-ASW 1.7 | ||
|incubation=Stage incubator (T+CO2) | |incubation=Stage incubator (T+CO2) | ||
Revision as of 16:08, 13 May 2014
|
Directions
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
| microscope | Olympus - BX 61, Inverted stand, motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC | ||
| objectives | |||
| illumination | |||
| detection | [[detection::point scanner, 4 confocal PMTs, two independent spectral detection channels, each one configured with a diffraction grating and variable slit for high-resolution wavelength separation and high-speed bandwidth selection, two filter based detection channels, T-PMT, dichromatic mirrors for excitation/emission splitting, one pinhole]] | ||
| reflectors | [[reflectors::Filter Cube 1. U-MWU2. BP330-385 BA420 DM400
Filter Cube 2. U-MWB2. BP460-490 BA520IF (High performance interference filter) DM500 | ||
| features | Objectives 40x 1.25 SIL and 60x 1.3 SIL are shared objectives and need to be booked separately via the LMF booking database!
Two independent and fully synchronized laser scanners for simultaneous laser stimulation and observation, laser stimulation can be performed using the imaging laser or a 405nm picosecond Diode laser (PicoQuant), sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, Kalman averaging, summarizing, scan zoom and rotation; advanced use of motorized stage due to MATL-software, which allows independent multi-point acquisition and tiling + some simple stitching"Objectives 40x 1.25 SIL and 60x 1.3 SIL are shared objectives and need to be booked separately via the LMF booking database! Two independent and fully synchronized laser scanners for simultaneous laser stimulation and observation, laser stimulation can be performed using the imaging laser or a 405nm picosecond Diode laser (PicoQuant), sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, Kalman averaging, summarizing, scan zoom and rotation; advanced use of motorized stage due to MATL-software, which allows independent multi-point acquisition and tiling + some simple stitching" cannot be used as a page name in this wiki. | ||
| software | FV10-ASW 1.7 | ||
| incubation | Stage incubator (T+CO2) | ||
| links |
| ||
| inv.nr. | MOZG0407421000 10.1.38.205 |
