H2 - Nikon STORM - TIRF

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|Facility-logo=MPI-CBG-Lmf-logo-big.png
 
|Facility-logo=MPI-CBG-Lmf-logo-big.png
 
|Institute-logo=Logo MPI-CBG.jpg
 
|Institute-logo=Logo MPI-CBG.jpg
|description=nikon TIRF / N-STORM setup.
+
|description=Nikon TIRF / N-STORM setup with two EM-CCDs for detection: at right port with a Hamamatsu W-View image splitter for simultaneous dual-color imaging; at left port with cylindrical lens for 3D-STORM; continuous laser-based autofocus PFS; strong 647nm (300mW) and 561nm (150mW) lasers;
|applications=N-STORM, fast TIRF
+
|applications=N-STORM, fast dual-color TIRF, long-term focus-stabilized low-light level imaging
 
|image=H2 - Nikon STORM - TIRF.jpg
 
|image=H2 - Nikon STORM - TIRF.jpg
 
|stand-name=Nikon - Eclipse Ti
 
|stand-name=Nikon - Eclipse Ti
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|link1=https://docs.google.com/spreadsheet/ccc?key=0AiAvGnTuH4LedFp2cllnYzdIdmItZWFYTGtDUFRXNFE&usp=sharing
 
|link1=https://docs.google.com/spreadsheet/ccc?key=0AiAvGnTuH4LedFp2cllnYzdIdmItZWFYTGtDUFRXNFE&usp=sharing
 
|inv=0  10.1.32.156
 
|inv=0  10.1.32.156
|facility_image=logo-lmf.jpg
 
|objectives=PlanApo 100x / 1.45 Oil TIRFM
 
|illumination=HBO<br>491 nm DPSS laser Cobolt<br>561 nm DPSS laser Cobolt
 
 
}}
 
}}

Revision as of 13:00, 13 May 2014

Suitable for N-STORM, fast dual-color TIRF, long-term focus-stabilized low-light level imaging.
H2 - Nikon STORM - TIRF.jpg


Directions

Logo MPI-CBG.jpg
MPI-CBG-Lmf-logo-big.pngMPI-CBG LMF (MPI-CBG, 036)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope Nikon - Eclipse Ti, Inverted stand, motorised stage
objectives
Nikon PlanApo 10x/0.45 DIC 0.17
Nikon PlanApo 60x/1.2 WI 0.15-0.18
Nikon Apo 60x/1.49 Oil Tirf 0.13-0.21
Nikon ApoVC 100x/1.4 Oil 0.17
Nikon Apo 100x/1.49 Oil DIC 0.13-0.20
illumination
Transmitted Light (Halogen)
Fluorescence (LED)
Laser Argon Multiline 458, 477, 488, 514 nm
Laser DPSS 561 nm
Laser DPSS 640 nm
detection Andor EM-CCD
reflectors image splitter:
features -
software NIS Elements
incubation Stage incubator (T+CO2) + objective heater
links
  • [ beam path]
  • [ advanced system check]
inv.nr. 0 10.1.32.156



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