H2 - Nikon STORM - TIRF

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Line 14: Line 14:
 
|stand-name=Nikon - Eclipse Ti
 
|stand-name=Nikon - Eclipse Ti
 
|stand=Inverted
 
|stand=Inverted
|microscope=motorised stage
+
|microscope=motorized stage; double-floor with two reflector-revolvers; manual 1.5x zoom lens; motorized TIRF-condenser with additional manual collimating lens
 
|obj1=Nikon PlanApo 10x/0.45 DIC 0.17
 
|obj1=Nikon PlanApo 10x/0.45 DIC 0.17
 
|obj2=Nikon PlanApo 60x/1.2 WI 0.15-0.18
 
|obj2=Nikon PlanApo 60x/1.2 WI 0.15-0.18
Line 25: Line 25:
 
|ill4=Laser DPSS 561 nm
 
|ill4=Laser DPSS 561 nm
 
|ill5=Laser DPSS 640 nm
 
|ill5=Laser DPSS 640 nm
|detection=Andor EM-CCD
+
|detection=at left camera port: Andor iXon EM+ DU-897 BV back-illuminated EM-CCD with cylindrical lens; dexel size of camera chip: 16um
|reflectors=image splitter:
+
at right camera port: Andor iXon3 DU-897 BV back-illuminated EM-CCD with 10 position filter wheel and Hamamtsu W-View image splitter; dexel size of camera chip: 16um
 +
|reflectors=Sutter filter wheel:
 +
 
 +
Hamamatsu W-View image splitter:
 
|features=-
 
|features=-
|software=NIS Elements
+
|software=NIS Elements with N-STORM module
|incubation=Stage incubator (T+CO2) + objective heater
+
|incubation=Cage incubator (T+CO2)
 
|link1=https://docs.google.com/spreadsheet/ccc?key=0AiAvGnTuH4LedFp2cllnYzdIdmItZWFYTGtDUFRXNFE&usp=sharing
 
|link1=https://docs.google.com/spreadsheet/ccc?key=0AiAvGnTuH4LedFp2cllnYzdIdmItZWFYTGtDUFRXNFE&usp=sharing
 
|inv=0  10.1.32.156
 
|inv=0  10.1.32.156
 
}}
 
}}

Revision as of 13:10, 13 May 2014

Suitable for N-STORM, fast dual-color TIRF, long-term focus-stabilized low-light level imaging.
H2 - Nikon STORM - TIRF.jpg


Directions

Logo MPI-CBG.jpg
MPI-CBG-Lmf-logo-big.pngMPI-CBG LMF (MPI-CBG, 036)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope Nikon - Eclipse Ti, Inverted stand, motorized stage; double-floor with two reflector-revolvers; manual 1.5x zoom lens; motorized TIRF-condenser with additional manual collimating lens
objectives
Nikon PlanApo 10x/0.45 DIC 0.17
Nikon PlanApo 60x/1.2 WI 0.15-0.18
Nikon Apo 60x/1.49 Oil Tirf 0.13-0.21
Nikon ApoVC 100x/1.4 Oil 0.17
Nikon Apo 100x/1.49 Oil DIC 0.13-0.20
illumination
Transmitted Light (Halogen)
Fluorescence (LED)
Laser Argon Multiline 458, 477, 488, 514 nm
Laser DPSS 561 nm
Laser DPSS 640 nm
detection at left camera port: Andor iXon EM+ DU-897 BV back-illuminated EM-CCD with cylindrical lens; dexel size of camera chip: 16um

at right camera port: Andor iXon3 DU-897 BV back-illuminated EM-CCD with 10 position filter wheel and Hamamtsu W-View image splitter; dexel size of camera chip: 16um

reflectors Sutter filter wheel:

Hamamatsu W-View image splitter:

features -
software NIS Elements with N-STORM module
incubation Cage incubator (T+CO2)
links
  • [ beam path]
  • [ advanced system check]
inv.nr. 0 10.1.32.156



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