H2 - Nikon STORM - TIRF

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|stand-name=Nikon - Eclipse Ti
 
|stand-name=Nikon - Eclipse Ti
 
|stand=Inverted
 
|stand=Inverted
|microscope=motorized stage; double-floor with two reflector-revolvers; manual 1.5x zoom lens; motorized TIRF-condenser with additional manual collimating lens
+
|microscope=motorized stage; double-floor with two reflector-revolvers; manual 1.5x zoom lens;<br>motorized TIRF-condenser with additional manual collimating lens
 
|obj1=Nikon PlanApo 10x/0.45 DIC 0.17
 
|obj1=Nikon PlanApo 10x/0.45 DIC 0.17
 
|obj2=Nikon PlanApo 60x/1.2 WI 0.15-0.18
 
|obj2=Nikon PlanApo 60x/1.2 WI 0.15-0.18
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|ill4=Laser DPSS 561 nm
 
|ill4=Laser DPSS 561 nm
 
|ill5=Laser DPSS 640 nm
 
|ill5=Laser DPSS 640 nm
|detection=at left camera port: Andor iXon EM+ DU-897 BV back-illuminated EM-CCD with cylindrical lens; dexel size of camera chip: 16um
+
|detection=at left camera port: Andor iXon EM+ DU-897 BV back-illuminated EM-CCD with cylindrical lens; dexel size of camera chip: 16um<br>at right camera port: Andor iXon3 DU-897 BV back-illuminated EM-CCD with 10 position filter wheel and Hamamtsu W-View image splitter; dexel size of camera chip: 16um
at right camera port: Andor iXon3 DU-897 BV back-illuminated EM-CCD with 10 position filter wheel and Hamamtsu W-View image splitter; dexel size of camera chip: 16um
+
|reflectors=Sutter filter wheel:<br>position 0: BrightLine 617/73, 1: Razor Edge LP488RS, 2: Razor Edge LP664RS, 3: BrightLine 447/60, 4: open, 5: Dual BrightLine 512/630 (512/23 + 630/91), 6: BrightLine 628/40, 7: BrightLine 525/30, 8: BrightLine 607/36, 9: BrightLine 520/15   
|reflectors=Sutter filter wheel:
+
position 0: BrightLine 617/73, 1: Razor Edge LP488RS, 2: Razor Edge LP664RS, 3: BrightLine 447/60, 4: open, 5: Dual BrightLine 512/630 (512/23 + 630/91), 6: BrightLine 628/40, 7: BrightLine 525/30, 8: BrightLine 607/36, 9: BrightLine 520/15   
+
  
 
Hamamatsu W-View image splitter:
 
Hamamatsu W-View image splitter:

Revision as of 14:28, 13 May 2014

Suitable for N-STORM, fast dual-color TIRF, long-term focus-stabilized low-light level imaging.
H2 - Nikon STORM - TIRF.jpg


Directions

Logo MPI-CBG.jpg
MPI-CBG-Lmf-logo-big.pngMPI-CBG LMF (MPI-CBG, 036)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope Nikon - Eclipse Ti, Inverted stand, motorized stage; double-floor with two reflector-revolvers; manual 1.5x zoom lens;
motorized TIRF-condenser with additional manual collimating lens"motorized stage; double-floor with two reflector-revolvers; manual 1.5x zoom lens;<br />motorized TIRF-condenser with additional manual collimating lens" cannot be used as a page name in this wiki.
objectives
Nikon PlanApo 10x/0.45 DIC 0.17
Nikon PlanApo 60x/1.2 WI 0.15-0.18
Nikon Apo 60x/1.49 Oil Tirf 0.13-0.21
Nikon ApoVC 100x/1.4 Oil 0.17
Nikon Apo 100x/1.49 Oil DIC 0.13-0.20
illumination
Transmitted Light (Halogen)
Fluorescence (LED)
Laser Argon Multiline 458, 477, 488, 514 nm
Laser DPSS 561 nm
Laser DPSS 640 nm
detection at left camera port: Andor iXon EM+ DU-897 BV back-illuminated EM-CCD with cylindrical lens; dexel size of camera chip: 16um
at right camera port: Andor iXon3 DU-897 BV back-illuminated EM-CCD with 10 position filter wheel and Hamamtsu W-View image splitter; dexel size of camera chip: 16um
reflectors Sutter filter wheel:
position 0: BrightLine 617/73, 1: Razor Edge LP488RS, 2: Razor Edge LP664RS, 3: BrightLine 447/60, 4: open, 5: Dual BrightLine 512/630 (512/23 + 630/91), 6: BrightLine 628/40, 7: BrightLine 525/30, 8: BrightLine 607/36, 9: BrightLine 520/15

Hamamatsu W-View image splitter:

features -
software NIS Elements with N-STORM module
incubation Cage incubator (T+CO2)
links
  • [ beam path]
  • [ advanced system check]
inv.nr. 0 10.1.32.156



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