H2 - Nikon STORM - TIRF
From BioDIP
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|ill5=Laser DPSS 640 nm | |ill5=Laser DPSS 640 nm | ||
|detection=at left camera port: Andor iXon EM+ DU-897 BV back-illuminated EM-CCD with cylindrical lens; dexel size of camera chip: 16um<br>at right camera port: Andor iXon3 DU-897 BV back-illuminated EM-CCD with 10 position filter wheel and Hamamtsu W-View image splitter; dexel size of camera chip: 16um | |detection=at left camera port: Andor iXon EM+ DU-897 BV back-illuminated EM-CCD with cylindrical lens; dexel size of camera chip: 16um<br>at right camera port: Andor iXon3 DU-897 BV back-illuminated EM-CCD with 10 position filter wheel and Hamamtsu W-View image splitter; dexel size of camera chip: 16um | ||
| − | |reflectors=Sutter filter wheel:<br>position 0: BrightLine 617/73, 1: Razor Edge LP488RS, 2: Razor Edge LP664RS, 3: BrightLine 447/60, 4: open, 5: Dual BrightLine 512/630 (512/23 + 630/91), 6: BrightLine 628/40, 7: BrightLine 525/30, 8: BrightLine 607/36, 9: BrightLine 520/15 | + | |reflectors=Microscope stand:<br>reflector revolver FL1 (lower floor):<br>position 1: L405 cube, 2: L470 cube, 3: L565 cube, 4: analyzer 1, 5: analyzer 2, 6: empty<br> reflector revolver FL2 (upper floor):<br> position 1: TIRF1 cube, 2: TIRFQ cube, 3: LGFP/mCherry cube, 4: YFP HQ cube, 5: L620 cube, 6: empty <br>Sutter filter wheel:<br>position 0: BrightLine 617/73, 1: Razor Edge LP488RS, 2: Razor Edge LP664RS, 3: BrightLine 447/60, 4: open, 5: Dual BrightLine 512/630 (512/23 + 630/91), 6: BrightLine 628/40, 7: BrightLine 525/30, 8: BrightLine 607/36, 9: BrightLine 520/15 <br>Hamamatsu W-View image splitter:<br>for bypass mode: silver mirror<br>for split mode: dichromatic mirror pair: 560 DCSP (transmission 500/100, reflection 615/90) and 560 DCLP (reflection 500/100, transmission 635/130), emission filters: ET BP 535/30, BL HC 628/40 |
| − | + | ||
| − | Hamamatsu W-View image splitter: | + | |
|features=- | |features=- | ||
|software=NIS Elements with N-STORM module | |software=NIS Elements with N-STORM module | ||
Revision as of 14:41, 13 May 2014
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Directions
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
| microscope | Nikon - Eclipse Ti, Inverted stand, motorized stage; double-floor with two reflector-revolvers; manual 1.5x zoom lens; motorized TIRF-condenser with additional manual collimating lens"motorized stage; double-floor with two reflector-revolvers; manual 1.5x zoom lens;<br />motorized TIRF-condenser with additional manual collimating lens" cannot be used as a page name in this wiki. | ||
| objectives | |||
| illumination | |||
| detection | at left camera port: Andor iXon EM+ DU-897 BV back-illuminated EM-CCD with cylindrical lens; dexel size of camera chip: 16um at right camera port: Andor iXon3 DU-897 BV back-illuminated EM-CCD with 10 position filter wheel and Hamamtsu W-View image splitter; dexel size of camera chip: 16um | ||
| reflectors | Microscope stand: reflector revolver FL1 (lower floor): position 1: L405 cube, 2: L470 cube, 3: L565 cube, 4: analyzer 1, 5: analyzer 2, 6: empty reflector revolver FL2 (upper floor): position 1: TIRF1 cube, 2: TIRFQ cube, 3: LGFP/mCherry cube, 4: YFP HQ cube, 5: L620 cube, 6: empty Sutter filter wheel: position 0: BrightLine 617/73, 1: Razor Edge LP488RS, 2: Razor Edge LP664RS, 3: BrightLine 447/60, 4: open, 5: Dual BrightLine 512/630 (512/23 + 630/91), 6: BrightLine 628/40, 7: BrightLine 525/30, 8: BrightLine 607/36, 9: BrightLine 520/15 Hamamatsu W-View image splitter: for bypass mode: silver mirror for split mode: dichromatic mirror pair: 560 DCSP (transmission 500/100, reflection 615/90) and 560 DCLP (reflection 500/100, transmission 635/130), emission filters: ET BP 535/30, BL HC 628/40 | ||
| features | - | ||
| software | NIS Elements with N-STORM module | ||
| incubation | Cage incubator (T+CO2) | ||
| links |
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| inv.nr. | 0 10.1.32.156 |
