Checklist for introducing new users to the Imaging Platform @ DZNE
From BioDIP
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| − | == | + | == First Contact == |
* If you are interested in using our facility please contact silke.white(a)dzne.de and ask for an introduction | * If you are interested in using our facility please contact silke.white(a)dzne.de and ask for an introduction | ||
| − | * In your email please describe your imaging project, | + | * In your email please describe your imaging project in as much detail as you can. As a guide, you can have a look at the [[New user project questions]]. You don't have to answer all the questions, but the more information you give, the easier it will be for the facility staff to help you. |
| − | ** | + | |
| + | |||
| + | == Admin introduction == | ||
| + | |||
| + | '' Estimated time: about 30 minutes '' | ||
| + | |||
| + | === Booking Database === | ||
| + | |||
| + | * General structure of database. | ||
| + | * Booking budgets, costs. | ||
| + | * How to book systems and assistance. | ||
| + | * How do blockings work. When and why might the facility ask users to give up their slot. | ||
| + | * How to report a problem. | ||
=== Booking Rules And Responsibilities === | === Booking Rules And Responsibilities === | ||
| − | * Delete and Cancel: the [[ | + | * Delete and Cancel: the [[DZNE - 24h rule|24h rule]]. |
| − | * The [[ | + | * The [[DZNE SIFFY|SIFFY]] procedure: how to offer and take a free slot. |
| − | * At the end of each session, check booking database: last user / not the last user --> what are the [[ | + | * At the end of each session, check booking database: last user / not the last user --> what are the [[The Last User' Responsibilities|responsibilities]]? |
| − | * [[ | + | * [[System Left On|Consequences]] for failing to switch off a system: extended booking + 15/30min assistant time |
| + | |||
* AND: "END OF SESSION" means: data saved, sample removed, objectives cleaned, software closed ... system is free for the next user!!! | * AND: "END OF SESSION" means: data saved, sample removed, objectives cleaned, software closed ... system is free for the next user!!! | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | == | + | === Safety and Waste === |
| + | |||
| + | * LMF is an S1 area - no S2 GMOs allowed! | ||
| + | * Especially: No eating and drinking in LMF, exception: LMF office | ||
| + | * Laser safety | ||
| + | * Waste: General (no S1, no glass, no sharpies); S1 (only solid, take liquid back to home lab); Glass (yellow bins at systems are S1 glass waste); Sharpies (for sharpies only, are S1) | ||
| + | |||
| + | |||
| + | === General === | ||
| + | |||
| + | * Sign the user into the IP-user mailing list | ||
| + | |||
| + | * Explain BioDIP Wiki! | ||
| + | |||
| + | |||
| + | == Theoretical Background (might be possible without system time) == | ||
| + | |||
| + | * Background to the techniques that will be used with the system, eg. contrasting techniques, fluorescence, beampath, pinhole, detector etc. | ||
| + | |||
| + | |||
| + | == On The System == | ||
=== First Introduction === | === First Introduction === | ||
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* Introduction to hardware: how to switch on/off. | * Introduction to hardware: how to switch on/off. | ||
| − | * Introduction to software: how to properly acquire an image | + | * Introduction to software: how to properly acquire an image, z-stack, time series (it might sometimes not be possible to cover all of that in the 1st intro). |
* Data handling: explain about original file formats and metadata; advantages/disadvantages of storing data on either hard drive or fileserver | * Data handling: explain about original file formats and metadata; advantages/disadvantages of storing data on either hard drive or fileserver | ||
| − | * Data safety: Point out that the | + | * Data safety: Point out that the LMF holds no responsibility for data whatsoever! |
| − | '''Important:''' in order to keep the disk free for imaging - data stored on hard drives will be deleted on an irregular basis. | + | '''Important:''' in order to keep the disk free for imaging - data stored on hard drives older than 1 month will be deleted on an irregular basis without warning. |
* Safety regulations: laser safety, S1 safety, correct use of wastebins. | * Safety regulations: laser safety, S1 safety, correct use of wastebins. | ||
* How to clean objectives. | * How to clean objectives. | ||
| + | |||
=== Follow Up Introduction === | === Follow Up Introduction === | ||
| Line 45: | Line 75: | ||
* Help user to set up the system for their own imaging experiment. | * Help user to set up the system for their own imaging experiment. | ||
* Stay with user until he/she feels save to use the system independently. | * Stay with user until he/she feels save to use the system independently. | ||
| − | + | ||
| + | |||
| + | '''Users are strongly encouraged to contact the IP- team about questions / problems / changes in their imaging projects at any time ''' | ||
Latest revision as of 11:00, 28 October 2013
Contents |
[edit] First Contact
- If you are interested in using our facility please contact silke.white(a)dzne.de and ask for an introduction
- In your email please describe your imaging project in as much detail as you can. As a guide, you can have a look at the New user project questions. You don't have to answer all the questions, but the more information you give, the easier it will be for the facility staff to help you.
[edit] Admin introduction
Estimated time: about 30 minutes
[edit] Booking Database
- General structure of database.
- Booking budgets, costs.
- How to book systems and assistance.
- How do blockings work. When and why might the facility ask users to give up their slot.
- How to report a problem.
[edit] Booking Rules And Responsibilities
- Delete and Cancel: the 24h rule.
- The SIFFY procedure: how to offer and take a free slot.
- At the end of each session, check booking database: last user / not the last user --> what are the responsibilities?
- Consequences for failing to switch off a system: extended booking + 15/30min assistant time
- AND: "END OF SESSION" means: data saved, sample removed, objectives cleaned, software closed ... system is free for the next user!!!
[edit] Safety and Waste
- LMF is an S1 area - no S2 GMOs allowed!
- Especially: No eating and drinking in LMF, exception: LMF office
- Laser safety
- Waste: General (no S1, no glass, no sharpies); S1 (only solid, take liquid back to home lab); Glass (yellow bins at systems are S1 glass waste); Sharpies (for sharpies only, are S1)
[edit] General
- Sign the user into the IP-user mailing list
- Explain BioDIP Wiki!
[edit] Theoretical Background (might be possible without system time)
- Background to the techniques that will be used with the system, eg. contrasting techniques, fluorescence, beampath, pinhole, detector etc.
[edit] On The System
[edit] First Introduction
Usually WITHOUT own sample!!!
Estimated time: system dependent, but usually around 2hours
- Introduction to hardware: how to switch on/off.
- Introduction to software: how to properly acquire an image, z-stack, time series (it might sometimes not be possible to cover all of that in the 1st intro).
- Data handling: explain about original file formats and metadata; advantages/disadvantages of storing data on either hard drive or fileserver
- Data safety: Point out that the LMF holds no responsibility for data whatsoever!
Important: in order to keep the disk free for imaging - data stored on hard drives older than 1 month will be deleted on an irregular basis without warning.
- Safety regulations: laser safety, S1 safety, correct use of wastebins.
- How to clean objectives.
[edit] Follow Up Introduction
With own sample!!!
Estimated time: strongly project dependent, but 1-2 hours are usually a good estimate
- Recap contents of first intro
- Introduce user to things that couldn't be covered in the first session
- Help user to set up the system for their own imaging experiment.
- Stay with user until he/she feels save to use the system independently.
Users are strongly encouraged to contact the IP- team about questions / problems / changes in their imaging projects at any time
