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| SD4 - Andor Revolution WD Borealis Mosaic |
| Applications | Confocal imaging, photo-activation using 405 laser with high temporal and spatial resolution; fast imaging of large samples over a long period of time + |
|---|---|
| Building | MPI-CBG + |
| Description | Fast, "real-time" spinning disc confocal imaging with photo-activation capability. Fast imaging of large field of view and large tile scans with even illumination. + |
| Detection | Spinning disc scan head Yokogawa CSU-W1 (4000rpm) with two pinhole discs (50um and 25um) and simultaneous dual camera detection: Camera 1 (Master): Andor iXon 888 Ultra with fringe suppression; Camera 2 (Slave): Andor iXon 888 Ultra |
| Facility | MPI-CBG LMF + |
| Id | MPI- SD4 + |
| Ill | Transmitted Light (LED) + , Laser Diode 405 nm + , Laser DPSS 561 nm + , Fluorescence (LED) + , Laser Diode 445 nm + , Laser DPSS 514 nm + , Laser DPSS 726nm + , Laser DPSS 488 nm + , Laser DPSS 638nm + |
| Image | SD4 system.jpg + |
| Incubation | Cage incubator (T+CO2) + |
| Name | SD4 Andor Revolution WD Borealis Mosaic + |
| Obj | Olympus UPlan SApochromat 10x 0.4 + , Olympus UPlanSApo 30x 1,05 S + , Olympus UPLSAPO 40x 1.25 SIL + , Olympus UPLSAPO 60x 1.3 SIL + , Olympus UPlan SApochromat 100x 1.4 Oil + , Olympus UAPON 150x 1.45 TIRFM + |
| Reflectors | CSU-W scan head:<br /> 3 main DM: Tr … CSU-W scan head:<br /> 3 main DM: Tripple (for 445nm, 515nm, 640nm laser); Quad (405nm, 488nm, 561nm, 640nm); Penta (405nm, 488nm, 561nm, 640nm, 720nm)<br /> FW1 (Master): Pos(1) LP568, Pos(2) 525/50, Pos(3) 617/73, Pos(4) 607/36, Pos(5) 685/40, Pos(6) 785/62, Pos(7) pol, Pos(8) LP514, Pos(9) 542/27, Pos(10) 452/45 <br /> FW1 (Slave): Pos(1) 465/30, Pos(2) 541/30, Pos(3) 525/50, Pos(4) 479/40, Pos(5) pol, Pos(6) - Pos(10) empty<br /> reflectors in IX83 stand:<br /> DAPI set (Ex 377/50, DM 409, Em 447/60); GFP set (Ex 472/30, DM 495, Em 520/35); RFP set (Ex 562/40, DM 593, Em 624/40); CY5 set (Ex 628/40, DM 660, Em 692/40); Analyzer; DM for use with Mosaic 692/40); Analyzer; DM for use with Mosaic |
| Room | 036 + |
| Stand | Inverted + |
| Stand-details | Motorized with ZDC (hardware autofocus and focus search), Prior NanoScanZ (400um travel range), Prior ProScanIII xy scanning stage + |
| Stand-name | Olympus - IX83 + |
| System type | Real-Time Confocal + |
| Type | Microscope + |
| Has queryThis property is a special property in this wiki. | SD4 - Andor Revolution WD Borealis Mosaic + |
| Has improper value forThis property is a special property in this wiki. | Description + , Software + |
| Categories | Microscope + , Real-Time Confocal + , MPI-CBG LMF + |
| Modification dateThis property is a special property in this wiki. | 8 June 2020 09:59:40 + |
| PageThis property is a special property in this wiki. | SD4 - Andor Revolution WD Borealis Mosaic + |
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| SD4 - Andor Revolution WD Borealis Mosaic + | PageThis property is a special property in this wiki. |
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