ApoTome1 3rd floor upright CRTD (Zeiss)
It is designed for routine work on fixed samples. Optical sectioning can be obtained on fixed samples by the use of the Apotome.
Category
Widefield, ApoTome
Location
CRTD, 3rd floor, LMF CMCB, room 3.325
Suitable for Specimen
Suitable for Technique
Microscope Stand
Objectives
2.5x/0.12 Fluar, Air, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 8.7 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 176 2.5x/0.12 Zeiss |
| Order Number: | 420120-9901-000 |
10x/0.45 Plan-Apochromat, Air, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 2 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 83 10x/0.45 Zeiss |
| Order Number: | 420640-9800-000 |
20x/0.8 Plan-Apochromat, Air, DIC, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.55 mm |
| Nomarski Prism: | EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 84 20x/0.8 Zeiss |
| Order Number: | 420650-9901-000 |
40x/0.95 Plan-Apochromat, Air, DIC, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.25 mm |
| Nomarski Prism: | EC PN and Fluar 40/1,3 III; PA 40/0,95 III (426944) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 123 40x/0.95 Zeiss |
| Order Number: | 420660-9970-000 |
40x/0.75 Achroplan, Water, DIC, Zeiss
| Immersion: | Water |
| Working Distance: | 2.1 mm |
| Nomarski Prism: | Achroplan 40/0,75 W III (444451) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | CMCB 104 40x/0.75 Water dipping Zeiss |
| Order Number: | 440090 |
63x/1.2 C-Apochromat, Water, DIC, Zeiss
| Immersion: | Water |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.28 mm at cover glass 0.17 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | CMCB 93 63x/1.2 Water Zeiss |
| Order Number: | 440668 |
Condenser
Fluorescence Filter Cubes
motorized revolver
polarizer (set Analysator)
polarizer cube for DIC/Polarization
Zeiss, set Analysator
DAPI (set F46-000)
single cube for DAPI, Alexa 350
- BP 387/11 (BP 381-392), excitation filter, bandpass, AHF
- LP 400, dichromatic mirror, longpass, AHF
- BP 460/50 (BP 435-485), emission filter, bandpass, AHF
Zeiss, set F46-000
GFP (set F46-000)
single cube for Alexa488/GFP, FITC
- BP 470/40 (BP 450-490), excitation filter, bandpass, AHF
- LP 495, dichromatic mirror, longpass, AHF
- BP 525/36 (BP 507-543), emission filter, bandpass, AHF
Zeiss
Cy3 (set F46-004)
single cube for Alexa555/Cy3/RFP, TRITC
- BP 590/33 (BP 573-606), emission filter, bandpass, AHF
- LP 565, dichromatic mirror, longpass, AHF
- BP 545/25 (BP 533-558), excitation filter, bandpass, AHF
Zeiss, set F46-004
Cy5 (set F46-006)
single cube for Alexa633/Cy5
- BP 640/30 (BP 625-655), excitation filter, bandpass, Zeiss
- LP 660, dichromatic mirror, longpass, Zeiss
- BP 700/75 (BP 663-738), emission filter, bandpass, Zeiss
Zeiss, set F46-006
YFP (set F46-003)
single cube for YFP
- BP 500/20 (BP 490-510), excitation filter, bandpass, AHF
- LP 515, dichromatic mirror, longpass, Leica
- BP 535/30 (BP 520-550), emission filter, bandpass, AHF
Zeiss, set F46-003
GFP/RFP (set 24)
double cube for GFP/RFP
- BP 485/20; BP 578/14 (BP 475-495; BP 571-585), excitation filter, double bandpass, Zeiss
- BP 528/65, LP 600 (BP 495-560, LP 600), double dichromatic mirror, double bandpass, Zeiss
- BP 528/25; LP 610 (BP 515-540; LP 610), emission filter, double bandpass, Zeiss
Zeiss, set 24
Transmitted Illumination
Lamp (Halogen, 100W)
Fluorescence Illumination
HXP 120C
Zeiss, Metal halide lamp, 120W, integrated
Camera
Zeiss AxioCam MRc, Color CCD camera, dexel size 6.45 µm
| port | top port |
| magnification | 1x |
| bit depth | 12 bit |
| sensor size | 8.9 x 6.7 mm |
| maximum frame size | 1388 x 1040 dexel |
| maximum full chip frame rate | 12.86 fps |
| Serial number | 206081617 |
Zeiss AxioCam MRm, Monochrome CCD camera, dexel size 6.45 µm
| port | left side port |
| magnification | 1x |
| bit depth | 12 bit |
| sensor size | 8.9 x 6.7 mm |
| maximum frame size | 1388 x 1040 dexel |
| maximum full chip frame rate | 12.86 fps |
| Serial number | 112082065 |
XY Stage
Structured Illumination
ApoTome1, Zeiss
structured illumination for optical sectioning
Computer
Software
Additional Information