Biopolis Dresden Imaging Platform

ApoTome1 inverse BIOTEC (Zeiss)

Our Apotome microscope inverted is designed for routine work on fixed sample. Optical sectioning can be obtained on fixed samples by the use of the ApoTome.

Category

Widefield, ApoTome

Location

BIOTEC, 2nd floor, LMF CMCB, room 225

Suitable for Specimen
Fixed samples
Tissue sections
Suitable for Technique
Large area tile/mosaic scans
Multi wellplate imaging
Microscope Stand

Zeiss Axiovert 200M, inverted stand
motorized objective revolver, motorized z-focus

Objectives

2.5x/0.12 Fluar, Air, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 8.7 mm
Thread: M27
Tube Length: infinity
Objective ID: CMCB 202 2.5x/0.12 Zeiss
Order Number: 420120-9901-000
Inventory Number: 25177461

5x/0.12 A-Plan, Air, Ph0, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 10.1 mm
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: CMCB 92 5x/0.12 Ph0 Zeiss
Order Number: 441021-9910-000

10x/0.45 Plan-Apochromat, Air, DIC, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 2 mm
Nomarski Prism: Planapo 10/0,45 II (1045-073)
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: CMCB 121 10x/0.45 Zeiss
Order Number: 000000-1063-139

20x/0.8 Plan-Apochromat, Air, DIC, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 0.55 mm
Nomarski Prism: EC PN 10/0,30 I, 20/0,50 II, PA 20/0,80 II (426940)
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: CMCB 122 20x/0.8 Zeiss
Order Number: 440640-9903-000

40x/0.95 Plan-Apochromat, Air, DIC, Zeiss

Immersion: Air
Collar: coverslip correction
Coverslip Thickness: 0.17
Working Distance: 0.25 mm
Nomarski Prism: EC PN and Fluar 40/1,3 III; PA 40/0,95 III (426944)
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: CMCB 86 40x/0.95 Zeiss
Order Number: 440654-9902-000

63x/1.4 Plan-Apochromat, Oil, DIC, Zeiss

Immersion: Oil
Coverslip Thickness: 0.17
Working Distance: 0.19 mm
Nomarski Prism: Planapo 63x/1,40 III (1122-909)
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: CMCB 38 63x/1.4 Oil Zeiss
Order Number: 000000-1113-108

100x/1.45 Achroplan, Oil, DIC, Zeiss

Immersion: Oil
Coverslip Thickness: 0.17
Working Distance: 0.11 mm
Nomarski Prism: alpha Plan-Fluar 100/1,45 III (1131-718)
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: CMCB 97 100x/1.45 Oil Zeiss
Order Number: 000000-1084-514
Condenser

BF, DIC III, Ph1, Ph2, Ph3
NA 0.55, working distance 26 mm, motorized

Fluorescence Filter Cubes

motorized revolver

DAPI ()

single cube for Alexa 350, DAPI

  • BP 387/11 (BP 381-392), excitation filter, bandpass, AHF
  • LP 400, dichromatic mirror, longpass, Zeiss
  • BP 460/50 (BP 435-485), emission filter, bandpass, Zeiss

Zeiss

GFP (set F46-002)

single cube for Alexa488/GFP, FITC

  • BP 470/40 (BP 450-490), excitation filter, bandpass, Chroma
  • LP 495, dichromatic mirror, longpass, Chroma
  • BP 525/50 (BP 500-550), emission filter, bandpass, Chroma

NIKON, set F46-002

Cy3 (set 49004)

single cube for Alexa555/Cy3/RFP, TRITC

  • BP 545/25 (BP 533-558), excitation filter, bandpass, Chroma
  • LP 565, dichromatic mirror, longpass, Chroma
  • BP 605/70 (BP 570-640), emission filter, bandpass, Chroma

Zeiss, set 49004

Cy5 (set 49004)

single cube for Alexa633/Cy5

  • BP 625/50 (BP 600-650), excitation filter, bandpass
  • LP 665, dichromatic mirror, longpass
  • BP 690/50 (BP 665-715), emission filter, bandpass

Zeiss

Calibration (set 49004)

single cube for Alexa633/Cy5

Zeiss

Transmitted Illumination

Lamp (Halogen, 100W)

Fluorescence Illumination

HXP 120C

Zeiss, Metal halide lamp, 120W, integrated
Camera

Zeiss AxioCam MRc, Color CCD camera, dexel size 6.45 µm

port front port
magnification 0.6x
bit depth 12 bit
sensor size 8.9 x 6.7 mm
maximum frame size 1388 x 1040 dexel
maximum full chip frame rate 12.86 fps
Inventory number 175067
Serial number 243092653

Zeiss AxioCam MRm, Monochrome CCD camera, dexel size 6.45 µm

port left side port
magnification 1x
bit depth 12 bit
sensor size 8.9 x 6.7 mm
maximum frame size 1388 x 1040 dexel
maximum full chip frame rate 12.86 fps
Serial number 110060309
XY Stage

motorized stage

Märzhäuser, SCAN IM, S/N 03111993

Structured Illumination

ApoTome1, Zeiss

structured illumination for optical sectioning

Computer

HP Z820 Workstation

Windows 7 Ultimate (dual boot 32/64 bit) operating system

  • 8 x 8 GB RAM
  • Intel Xeon E5-2643 (2x) CPU
  • AMD FirePro V5900 (2GB) grafic card
  • 30" display
  • apot-inv-biotec
Software

Zeiss ZEN blue, V2012 1.1.2.0

  • 1205494619
  • Experiment Designer
  • Extended Focus
  • Image Analysis
  • Measurement
  • Multi channel acquisition
  • Tiles
  • Time Lapse
  • Z-stack

Zeiss Axiovision,

Additional Information
  • Inventory Number 121643000
  • SIP Number 46517
  • Contact for Service Raik Stäber (0151/42112278)
  • DRESDEN concept ID 1290