H4 - OMX Flex (GE)
The OMX-FLEX is primarily a structured illumination microscope (SIM), which works in several modes: 2D SIM, 3D SIM and TIRF SIM.
The microscope is equipped with 4x sCMOS cameras, 4 lasers: 405nm, 488nm, 561nm, 642nm, a set of filters suitable for DAPI/GFP/TRITC/CY5, and has photo-manipulation capabilities (FRAP and photo-activation).
The microscope has other imaging modalities:
- fast Widefield imaging
- ring TIRF
- line scanning confocal microscopy (IRIS) and enhanced confocal mode (EDGE)
- SMLM.
In addition to the list of objectives that are present by default at the microscope (see below), objectives such as 40x/1.25 Silicon might be used when using the widefield or confocal imaging modes.
Category
Super resolution, Structured Illumination (SIM)
Location
MPI-CBG, LMF MPI-CBG, room 036
Suitable for Specimen
Suitable for Technique
Microscope Stand
Objectives
60x/1.42 Plan Apo N, Oil, OLYMPUS
| Immersion: | Oil |
| Coverslip Thickness: | 0.17 |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 393 60x/1.42 Olympus |
| Order Number: | PLAPON60XOPSF |
60x/1.49 Apo N, Oil, OLYMPUS
| Immersion: | Oil |
| Collar: | temperature and coverslip correction |
| Coverslip Thickness: | 0.13-0.19 |
| Working Distance: | 0.1 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 394 60x/1.49 Olympus |
| Order Number: | APON60XOTIRF |
20x/0.8 U Plan Apo, Oil, OLYMPUS
| Immersion: | Oil |
| Coverslip Thickness: | - |
| Nomarski Prism: | A |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 144 20x/0.8 Olympus |
Condenser
Fluorescence Filter Cubes
slider
Polychroic filter B/G/R/FR ()
quad cube
- excitation filter, bandpass
- (BP 417-476), emission filter, bandpass
- (BP 486-490), excitation filter, bandpass
- (BP 500-550), emission filter, bandpass
- (BP 560-569), excitation filter, bandpass
- (BP 580-627), emission filter, bandpass
- (BP 637-643), excitation filter, bandpass
- (BP 655-705), emission filter, bandpass
GE
Emission filter set B/G/R/FR
quad cube for DAPI/GFP/RFP/Cy5
- BP 447/60, emission filter, bandpass
- BP 525/50, emission filter, bandpass
- BP 603/48, emission filter, bandpass
- BP 679/52, emission filter, bandpass
GE
Transmitted Illumination
LED (LED)
Fluorescence Illumination
405nm
Laser Diode, integrated
488
Diode Laser, integrated
561nm
Laser DPSS, integrated
640nm
Laser Diode, integrated
Camera
PCO Edge 4.2, Monochrome sCMOS camera, dexel size 6.5 µm
| port | Back port 1 - DAPI |
| magnification | 1.3x |
| bit depth | 16 bit |
| sensor size | 13.3 x 13.3 mm |
| maximum frame size | 2048 x 2048 dexel |
| maximum full chip frame rate | 100 fps |
| Serial number | 61006067 |
PCO Edge 4.2, Monochrome sCMOS camera, dexel size 6.5 µm
| port | Back port 1 - A488 |
| magnification | 1.3x |
| bit depth | 16 bit |
| sensor size | 13.3 x 13.3 mm |
| maximum frame size | 2048 x 2048 dexel |
| maximum full chip frame rate | 100 fps |
| Serial number | 61006048 |
PCO Edge 4.2, Monochrome sCMOS camera, dexel size 6.5 µm
| port | Back port 3 - A568 |
| magnification | 1.3x |
| bit depth | 16 bit |
| sensor size | 13.3 x 13.3 mm |
| maximum frame size | 2048 x 2048 dexel |
| maximum full chip frame rate | 100 fps |
| Serial number | 610066107 |
PCO Edge 4.2, Monochrome sCMOS camera, dexel size 6.5 µm
| port | Back port 4 - Cy5 |
| magnification | 1.3x |
| bit depth | 16 bit |
| sensor size | 13.3 x 13.3 mm |
| maximum frame size | 2048 x 2048 dexel |
| maximum full chip frame rate | 100 fps |
| Serial number | 61006046 |
XY Stage
Hardware Autofocus
GE Ultimate focus
Scan Head
Structured Illumination
OMX, GE
structured illumination for superresolution
Extra Photo Manipulation
Incubation
Computer
Software
Additional Information