SD2 - Andor Spinning Disc FRAPPA (Andor)
Fast, "real-time" confocal imaging with FRAPPA capability.
Illumination equipment: Cairn OptoLED lite, 405nm diode laser (100mW) + 488 nm solid-state laser Coherent Sapphire (50mW) + 561 nm solid-state laser Cobolt Jive (50mW) + 640 nm solid-state laser (100mW).
Link to pixel sizes.
Category
Spinning disk
Location
MPI-CBG, LMF MPI-CBG, room 038B
Microscope Stand
Objectives
10x/0.4 U Plan SApo, Air, OLYMPUS
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 3.1 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 194 10x/0.4 Olympus |
| Order Number: | UPLSAPO10X2 |
| Inventory Number: | 4077990000 |
40x/1.15 U Plan Apo, Water, OLYMPUS
| Immersion: | Water |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.13-0.25 |
| Working Distance: | 0.25 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 195 40x/1.15 Olympus |
| Order Number: | UAPON40XW340 |
| Inventory Number: | 4092840000 |
60x/0.9 U Plan FL N, Air, OLYMPUS
| Immersion: | Air |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.11-0.23 |
| Working Distance: | 0.2 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 189 60x/0.9 Olympus |
| Order Number: | UPLFLN60X |
| Inventory Number: | 4078630000 |
60x/1.2 U Plan SApo, Water, OLYMPUS
| Immersion: | Water |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.13-0.21 |
| Working Distance: | 0.28 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 159 60x/1.2 Olympus |
| Order Number: | UPLSAPO60XW |
| Inventory Number: | 4087650000 |
60x/1.35 U Plan SApo, Oil, OLYMPUS
| Immersion: | Oil |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.15 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 161 60x/1.35 Olympus |
| Order Number: | UPSLAPO60X0 |
100x/1.4 U Plan SApo, Oil, OLYMPUS
| Immersion: | Oil |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.13 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 198 100x/1.4 Olympus |
| Order Number: | UPLSAPO100XO |
| Inventory Number: | 4064660000 |
Condenser
Fluorescence Filter Cubes
motorized revolver
EMPTY ()
empty for through position
- empty
polarizer ()
empty for through position
GFP/RFP ()
double cube for Alexa488/GFP, Alexa555/Cy3/RFP
eGFP ()
single cube for Alexa488/GFP
RFP ()
single cube for Alexa 568, Alexa555/Cy3/RFP
DAPI ()
single cube for Alexa 405, DAPI
Additional Filters
Alexa 405, Alexa 488, Alexa 568, Alexa 647, Alexa 488, Alexa 647, DAPI, Alexa 488
- BP 440/40; BP 521/21; BP 607/34; BP 700/45 (BP 420-460; BP 511-532; BP 590-624; BP 678-723), emission filter, quadro bandpass
- LP 488 Razor edge, emission filter, longpass
- BP 700/75, emission filter, bandpass
- LP 568 Razor edge, emission filter, longpass
- LP 635, emission filter, longpass
- BP 445/45, emission filter, bandpass
- empty
- Polariser
- BP 525/30, emission filter, bandpass
- BP 607/36, emission filter, bandpass
CFP
- empty
- BP 470/22, emission filter, bandpass
- Polariser
Transmitted Illumination
LED (LED)
Fluorescence Illumination
HBO
Mercury arc lamp, 100W
405nm
Laser Diode, integrated
488nm
Laser DPSS, integrated
561nm
Laser DPSS, integrated
640nm
Laser DPSS
Camera
Andor Clara cooled interline CCD, Monochrome CCD camera, dexel size 6.45 µm
| port | Left side port |
| magnification | 0.95x |
| bit depth | 16 bit |
| sensor size | 8.98 x 6.71 mm |
| maximum frame size | 1392 x 1040 dexel |
| maximum full chip frame rate | 11 fps |
Andor iXON 897 EMCCD, Monochrome EMCCD camera, dexel size 16 µm
| port | Left side port |
| bit depth | 14 bit |
| sensor size | 8.2 x 8.2 mm |
| maximum frame size | 512 x 512 dexel |
| maximum full chip frame rate | 35-549 fps |
XY Stage
Hardware Autofocus
OLYMPUS IX 81 ZDC
Scan Head
Incubation
Additional Information