Biopolis Dresden Imaging Platform

CFCI-WF 01 ApoTome I (Zeiss)

Widefield System with ApoTome

This microscope is designed for routine work on fixed or living samples. Optical sectioning can be obtained on fixed samples by using the ApoTome (structured illumination). The microscope is also capable for brightfield including contrast techniques like phase contrast, darkfield and polarized light. Furthermore the second camera (colour) allows the imaging of histological stained samples.

Category

Widefield, ApoTome

Location

MTZ, LMF & EMF CFCI, room A.20.055

Suitable for Specimen
Fixed samples
Live samples
Tissue sections
Suitable for Technique
Multi wellplate imaging
Large area tile/mosaic scans
Multiple positions
Microscope Stand

Zeiss Axio Observer.Z1, inverted stand
motorized objective revolver, motorized z-focus

Objectives

10x/0.3 EC Plan-Neofluar, Air, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 5.2 mm
Thread: M27
Tube Length: infinity
Objective ID: MTZ Z_10x_0.3_01 10x/0.3 Zeiss
Order Number: 420340-9901-000

20x/0.5 EC Plan-Neofluar, Air, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 2 mm
Thread: M27
Tube Length: infinity
Objective ID: MTZ Z_20x_0.5_01 20x/0.5 Zeiss
Order Number: 420350-9900-000

40x/0.75 EC Plan-Neofluar, Air, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 0.71 mm
Thread: M27
Tube Length: infinity
Objective ID: MTZ Z_40x_0.75_01 40x/0.75 Zeiss
Order Number: 420360-9900-000

63x/1.4 Plan-Apochromat, Oil, Zeiss

Immersion: Oil
Coverslip Thickness: 0.17
Working Distance: 0.19 mm
Thread: M27
Tube Length: infinity
Objective ID: MTZ Z_63x_1.4_02 63x/1.4 Zeiss
Order Number: 420780-9900-000

20x/0.3 Achrostigmat, Air, Ph1, Zeiss

Immersion: Air
Thread: RMS (20.32×0.71 mm)
Tube Length: infinity
Objective ID: MTZ Z_20x_0.3_01 20x/0.3 Zeiss
Condenser

H/DIC, Ph1, Ph2, Ph3, VAREL
NA 0.55, working distance 26 mm, manual

Fluorescence Filter Cubes

motorized revolver

DAPI (set 488049-9901-000)

single cube for DAPI

  • G 365, excitation filter, bandpass, Zeiss
  • LP 395, dichromatic mirror, longpass, Zeiss
  • BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss

, set 488049-9901-000

GFP (set 489038-9901-000)

single cube for Alexa488/GFP, CFP, FITC

  • BP 470/40 (BP 450-490), excitation filter, bandpass, Zeiss
  • LP 495, dichromatic mirror, longpass, Zeiss
  • BP 525/50 (BP 500-550), emission filter, bandpass, Zeiss

, set 488049-9901-000, set 489038-9901-000

Cy3 (set 489043-9901-000)

single cube for Alexa555/Cy3/RFP, DsRed

  • BP 550/25 (BP 525-575), excitation filter, bandpass, Zeiss
  • LP 570, dichromatic mirror, longpass, Zeiss
  • BP 605/70 (BP 570-640), emission filter, bandpass, Zeiss

, set 488049-9901-000, set 489038-9901-000, set 489043-9901-000

Transmitted Illumination

Lamp (HAL 100, 12V, 5600 K)

Fluorescence Illumination

HXP 120C

Zeiss, Metal halide lamp, 120W, integrated
Camera

Zeiss AxioCam MRm, Monochrome CCD camera, dexel size 6.45 µm

port left side port
magnification 1x
bit depth 12 bit
sensor size 8.9 x 6.7 mm
maximum frame size 1388 x 1040 dexel
maximum full chip frame rate 12.86 fps
Serial number 112082065

Zeiss AxioCam MRc, Color CCD camera, dexel size 6.45 µm

port right side port
bit depth 12 bit
sensor size 8.9 x 6.7 mm
maximum frame size 1388 x 1040 dexel
maximum full chip frame rate 12.86 fps
XY Stage

motorized stage

Zeiss Scanningtisch 130x85 motP; CAN (D) with

  • universal holder K (slides, dishes 24-68mm)
  • universal holder K-X (for multiwellplates)
  • heating insert P-Set 2000 (Pecon)
Structured Illumination

ApoTome1, Zeiss

structured illumination for optical sectioning

Computer

Windows 7 Professional (64 bit) operating system

  • 2 x 8 GB RAM
  • Intel Core i5-4670 CPU
  • NVIDIA GeForce 8500 GT grafic card
  • 30" display
Additional Equipment

Pecon Heating Insert P-Set 2000
for dishes and slides

 

Additional Information
  • Inventory Number 0081389/00
  • Date of Installation 30.08.2010
  • End of Warranty 30.08.2012
  • Service Visits Upgrades 9/2014; 02/2016