Biopolis Dresden Imaging Platform

Versatile laser-based cell manipulator.

Maghelli N, Tolić-Nørrelykke IM

Here we describe a two-photon microscope and laser ablation setup combined with optical tweezers. We tested the setup on the fission yeast Schizosaccharomyces pombe, a commonly used model organism. We show that long-term imaging can be achieved without significant photo-bleaching or damage of the sample. The setup can precisely ablate sub-micrometer structures, such as microtubules and mitotic spindles, inside living cells, which remain viable after the manipulation. Longer exposure times lead to ablation, while shorter exposures lead to photo-bleaching of the target structure. We used optical tweezers to trap intracellular particles and to displace the cell nucleus. Two-photon fluorescence imaging of the manipulated cell can be performed simultaneously with trapping. The combination of techniques described here may help to solve a variety of problems in cell biology, such as positioning of organelles and the forces exerted by the cytoskeleton.

Taken form Maghelli et al, 2008.
  • J Biophotonics 2008 Sep;1(4):299-309
  • 2008
  • Imaging Technologies Development
  • 19343653
  • PubMed

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