Biopolis Dresden Imaging Platform

Electron tomography of microtubule end-morphologies in C. elegans embryos.

O'Toole E, Müller-Reichert T

In this chapter we describe the preparation of early mitotic C. elegans embryos for the tomographic reconstruction of end-morphologies of spindle microtubules. Early embryos are prepared by high-pressure freezing and freeze-substitution for thin-layer embedding in Epon/Araldite. We further describe data acquisition, tomographic reconstruction, and 3-D modeling of microtubules in serially sectioned mitotic spindles. The presented techniques are applicable to other model systems.

Taken from O’Toole et al, 2009.
  • Methods Mol. Biol. 2009;545:135-44
  • 2009
  • Imaging Technologies Development
  • 19475386
  • PubMed

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