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Amphipathic DNA origami nanoparticles to scaffold and deform lipid membrane vesicles.

Czogalla A, Kauert DJ, Franquelim HG, Uzunova V, Zhang Y, Seidel R, Schwille P

We report a synthetic biology-inspired approach for the engineering of amphipathic DNA origami structures as membrane-scaffolding tools. The structures have a flat membrane-binding interface decorated with cholesterol-derived anchors. Sticky oligonucleotide overhangs on their side facets enable lateral interactions leading to the formation of ordered arrays on the membrane. Such a tight and regular arrangement makes our DNA origami capable of deforming free-standing lipid membranes, mimicking the biological activity of coat-forming proteins, for example, from the I-/F-BAR family.

Fig.1 taken from Czogalla et al, 2015.
  • Angew. Chem. Int. Ed. Engl. 2015 May 26;54(22):6501-5
  • 2015
  • Biophysics
  • 25882792
  • PubMed

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