Checklist for introducing new users to the MPI-CBG Light Microscopy Facility

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Contents

First Contact

  • Send user link to New user project questions. Ask the user to send all relevant answers to lmf(at)mpi-cbg.de ideally before the first face to face meeting. (It might not make sense for every user to answer every single question).
  • Arrange a personal meeting with the new user and one or more members of the lmf team: discussion about imaging project and agreement on the most suitable system(s) using info from new user questions.
  • Point out main contact details: lmf(at)mpi-cbg.de or lmf-helpline phone 210-2345.
  • Avoid situations where a new user books a system at short notice and expects to get an introduction all done in 1 or 2 hours with no prior meeting/discussion about their project.


Administration (no system time needed)

Estimated time: about 30 minutes

Booking Database

  • General structure of database.
  • Booking budgets, costs.
  • How to book systems and assistance (combined and separately).
  • How do blockings work. When and why might the LMF ask users to give up their slot. Make user be aware of and respect the student's system checks!
  • How to use the "Report" function.


Booking Rules And Responsibilities

  • Delete and Cancel: the 24h rule.
  • The SIFFY procedure: how to offer and take a free slot.
  • At the end of each session, check booking database: last user / not the last user --> what are the responsibilities?
  • Consequences for failing to switch off a system: extended booking + 15/30min assistant time
  • AND: "END OF SESSION" means: data saved, sample removed, objectives cleaned, software closed ... system is free for the next user!!!



Theoretical Background (might be possible without system time)

  • Background to the techniques that will be used with the system, eg. contrasting techniques, fluorescence, beampath, pinhole, detector etc.


On The System

First Introduction

Usually WITHOUT own sample!!!

Estimated time: system dependent, but usually around 2hours

  • Introduction to hardware: how to switch on/off.
  • Introduction to software: how to properly acquire an image, z-stack, time series (it might sometimes not be possible to cover all of that in the 1st intro).
  • Data handling: explain about original file formats and metadata; advantages/disadvantages of storing data on either hard drive or fileserver
  • Data safety: Point out that the LMF holds no responsibility for data whatsoever!

Important: in order to keep the disk free for imaging - data stored on hard drives will be deleted on an irregular basis without warning.

  • Safety regulations: laser safety, S1 safety, correct use of wastebins.
  • How to clean objectives.


Follow Up Introduction

With own sample!!!

Estimated time: strongly project dependent, but 1-2 hours are usually a good estimate

  • Recap contents of first intro
  • Introduce user to things that couldn't be covered in the first session
  • Help user to set up the system for their own imaging experiment.
  • Stay with user until he/she feels save to use the system independently.
  • Invite user to contact the lmf team about questions / problems / changes in their imaging projects at any time.
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