Electron Microscopy Facility (EMF)
Max Planck Institute of Molecular Cell Biology and Genetics
We offer the technology, expertise and resources to study biological samples in the range of individual molecules to organoids and tissues. Our routine techniques include negative staining and resin embedding for investigations conducted at room temperature. Additionally, we are equipped to analyze vitrified samples at the near-native state using both fluorescence light and electron microscopy. While our facility specializes in transmission electron microscopy (TEM), we are currently expanding our capabilities to include large-volume acquisitions through scanning electron microscopy (SEM). Furthermore, we are actively developing workflows for correlative light and electron microscopy (CLEM) at both 2D and 3D scales. We are happy to discuss your project and collaborate to find the best possible solution for your research needs.
© MPI-CBG
Techniques
- negative staining of small samples and biomolecules
- Immobilization of cells and tissues through aldehyde crosslinking
- cryo-immobilization of cells and tissues via plunge or high-pressure freezing
- Immunolabelling (pre- and post-embedding) of endogenous proteins on resin- or cryosections
Specialities
- TEM in 2D and 3D (tomography
- SEM in 2D and 3D (FIB-SEM and SBF-SEM
- (cryo) Fluorescence light microscopy on bright field and confocal laser scanning microscopes
- (cryo) CLEM in 2D and 3D
- In Addition, we help users with data curation, analysis and visualization.
Team
© MPI-CBG
Felix Lange
Facility Leader
© BioDIP
Tobias Fürstenhaupt
EM Specialist
© BioDIP
Matthias Pöge
EM Specialist
© MPI-CBG
Jana Meissner
Technical Assistant
© MPI-CBG
Michaela Yuan
Technical Assistant
© MPI-CBG
Weihua Leng
Technical Assistant
Visitor's Address
Max Planck Institute of Molecular Cell Biology and Genetics
Electron Microscopy Facility
Pfotenhauerstrasse 108
01307 Dresden
Germany